Horizontal method for detection and enumeration of Campylobacter spp. - Part 1: Detection method
ISO 10272-1 (2017) method: Campylobacter
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Qualitative process control with RM-CJ
- Use a quantitative reference material (RM-CJ) with a known number of Campylobacter jejuni.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (RM-CJ) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at (41,5 ± 1) °C for (44 ± 4) h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: Greyish, flat and moist colonies, sometimes with metallic sheen.

Best Practice
Singl strain te be used for the detection and enumeration of Campylobacter spp.
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | WDCM number | NCCB number | |
---|---|---|---|---|---|
Single-strain reference material | Campylobacter jejuni | 00005 | 100333 | ||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |