Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique
Qualitative process control with RM-CJ
- Use a quantitative reference material (RM-CJ) with a known number of Campylobacter jejuni.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (RM-CJ) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at (41,5 ± 1) °C for 24h to 48 h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: Growth.
Process control detection method