ISO 22964 (2017) method: Cronobacter spp

Process control Food chain

Horizontal method for the detection of Cronobacter spp.

  • Quantitative process control with RM-CS

    • Growth. The characteristic reaction of on CCI
    Characteristic reaction: Growth.

    Best Practice

    The single strain RM-CS is used for the detection of Cronobacter sakazaki

    1. Use a quantitative reference material (RM-CS) with a known number of Cronobacter sakazakii.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (CCI) by spread plate method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (41,5 ± 1) °C for (24 ± 2) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Growth.

    Kind of reference material Biosisto product for this control method Control Strain WDCM number
    Single-strain reference material
    RM-CS
    		 Cronobacter sakazakii 00214
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.