ISO 6579-1 (2017) method: Salmonella

Process control Food chain

Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.

  • 1 Qualitative process control with CRM-SAG

    • colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium. The characteristic reaction of on XLD
    Characteristic reaction: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Best Practice

    The single strain CRM-SAG Salmonella agona is an atypical food related Salmonella. Salmonella agona is lactose positive. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)

    Process control detection method

    1. Use a quantitative reference material (CRM-SAG) with a known number of Salmonella enterica subsp. enterica serotype Agona.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use.
    4. In the case of qualitative operations the contamination level of the reference material (CRM-SAG) must be representative of the limit of detection.
    5. At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
    6. Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
    7. Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
    8. The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material
    CRM-SAG
    		 Salmonella enterica subsp. enterica serotype Agona 100283
    The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025.

  • 2 Qualitative process control with CRM-SE

    • colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium. The characteristic reaction of on XLD
    Characteristic reaction: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Best Practice

    The single strain CRM-SE Salmonella enteritidis is a typical food related Salmonella. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)

    Process control detection method

    1. Use a quantitative reference material (CRM-SE) with a known number of Salmonella enteritica subsp. enterica serotype Enteritidis.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use.
    4. In the case of qualitative operations the contamination level of the reference material (CRM-SE) must be representative of the limit of detection.
    5. At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
    6. Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
    7. Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
    8. The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material
    CRM-SE
    		 Salmonella enteritica subsp. enterica serotype Enteritidis 100284
    The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025.

  • 3 Qualitative process control with RM-SABAE

    • colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium. The characteristic reaction of on XLD
    Characteristic reaction: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Best Practice

    The single strain RM-SABAE is used for the enumeration of Salmonella. The single strain RM-SABAE Salmonella abaetetuba is not related to food. Salmonella abaetetuba will not normally be found in food products.

    Process control detection method

    1. Use a quantitative reference material (RM-SABAE) with a known number of Salmonella enterica subsp. enterica serotype Abaetetuba.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use.
    4. In the case of qualitative operations the contamination level of the reference material (RM-SABAE) must be representative of the limit of detection.
    5. At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
    6. Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
    7. Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
    8. The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material
    RM-SABAE
    		 Salmonella enterica subsp. enterica serotype Abaetetuba 100616
    The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025.

  • 4 Qualitative process control with RM-ST

    • colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium. The characteristic reaction of on XLD
    Characteristic reaction: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Best Practice

    The single strain RM-ST Salmonella typhimurium is a typical food related Salmonella. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)

    Process control detection method

    1. Use a quantitative reference material (RM-ST) with a known number of Salmonella enterica subsp. Enterica serotype Typhimurium.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use.
    4. In the case of qualitative operations the contamination level of the reference material (RM-ST) must be representative of the limit of detection.
    5. At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
    6. Incubate at 37 °C ± 1 °C for 37 °C ± 1 °C.
    7. Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
    8. The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Kind of reference material Biosisto product for this control method Control Strain WDCM number
    Single-strain reference material
    RM-ST
    		 Salmonella enterica subsp. Enterica serotype Typhimurium 00031
    The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025.

  • 5 Qualitative process control with RM-STRAN

    • colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium. The characteristic reaction of on XLD
    Characteristic reaction: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Best Practice

    The single strain RM-STRAN Salmonella tranaroa is not related to food. Salmonella tranaroa will not normally be found in food products.

    Process control detection method

    1. Use a quantitative reference material (RM-STRAN) with a known number of Salmonella enterica subsp. Salamae serotype Tranoroa.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use.
    4. In the case of qualitative operations the contamination level of the reference material (RM-STRAN) must be representative of the limit of detection.
    5. At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
    6. Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
    7. Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
    8. The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material
    RM-STRAN
    		 Salmonella enterica subsp. Salamae serotype Tranoroa 100480
    The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025.