Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
ISO 6579-1 (2017) method: Salmonella
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1 Qualitative process control with CRM-SAG
- Use a quantitative reference material (CRM-SAG) with a known number of Salmonella enterica subsp. enterica serotype Agona.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (CRM-SAG) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.
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2 Qualitative process control with CRM-SE
- Use a quantitative reference material (CRM-SE) with a known number of Salmonella enteritica subsp. enterica serotype Enteritidis.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (CRM-SE) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.
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3 Qualitative process control with RM-SABAE
- Use a quantitative reference material (RM-SABAE) with a known number of Salmonella enterica subsp. enterica serotype Abaetetuba.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (RM-SABAE) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.
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4 Qualitative process control with RM-ST
- Use a quantitative reference material (RM-ST) with a known number of Salmonella enterica subsp. Enterica serotype Typhimurium.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (RM-ST) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at 37 °C ± 1 °C for 37 °C ± 1 °C.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.
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5 Qualitative process control with RM-STRAN
- Use a quantitative reference material (RM-STRAN) with a known number of Salmonella enterica subsp. Salamae serotype Tranoroa.
- Defrost the material at room temperature for 30 minutes.
- Mix well before use.
- In the case of qualitative operations the contamination level of the reference material (RM-STRAN) must be representative of the limit of detection.
- At an average contamination level of 5 cfu per control sample, the probability of using a coincidentally uninfected (negative) sample according to the Poisson spread is less than 1%.
- Incubate at 37 °C ± 1 °C for 24 h ± 3 h.
- Determine the absence or presence of the microorganism and register your finding in a qualitative control chart.
- The characteristic reaction is: colonies with black centre and a lightly transparent zone of reddish colour due to the colour change of the medium.

Best Practice
The single strain CRM-SAG Salmonella agona is an atypical food related Salmonella. Salmonella agona is lactose positive. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | NCCB number | ||
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Single-strain reference material | Salmonella enterica subsp. enterica serotype Agona | 100283 | |||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |

Best Practice
The single strain CRM-SE Salmonella enteritidis is a typical food related Salmonella. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | NCCB number | ||
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Single-strain reference material | Salmonella enteritica subsp. enterica serotype Enteritidis | 100284 | |||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |

Best Practice
The single strain RM-SABAE is used for the enumeration of Salmonella. The single strain RM-SABAE Salmonella abaetetuba is not related to food. Salmonella abaetetuba will not normally be found in food products.
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | NCCB number | ||
---|---|---|---|---|---|
Single-strain reference material | Salmonella enterica subsp. enterica serotype Abaetetuba | 100616 | |||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |

Best Practice
The single strain RM-ST Salmonella typhimurium is a typical food related Salmonella. This Salmonella can be found in food products. If you want to avoid cross contamination, choose a not food related Salmonella like Salmonella tranaroa (RM-STRAN) or Salmonella abaetetuba (RM-SABAE)
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | WDCM number | ||
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Single-strain reference material | Salmonella enterica subsp. Enterica serotype Typhimurium | 00031 | |||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |

Best Practice
The single strain RM-STRAN Salmonella tranaroa is not related to food. Salmonella tranaroa will not normally be found in food products.
Process control detection method
Kind of reference material | Biosisto product for this control method | Control Strain | NCCB number | ||
---|---|---|---|---|---|
Single-strain reference material | Salmonella enterica subsp. Salamae serotype Tranoroa | 100480 | |||
The Biosisto reference material in this table is used in the best practice for Qualitative process control according ISO 17025. |