ISO 13720 (2010) method: Pseudomonas spp.

Process control Meat and meat products

Enumeration of presumptive Pseudomonas spp.

  • 1 Quantitative process control with CRM-PF

  • Growth. The characteristic reaction of  on CFC
    Characteristic reaction: Growth.

    Best Practice

    The single strain CRM-PF is used for the enumeration of Pseudomonas spp.

    1. Use a quantitative reference material (CRM-PF) with a known number of Pseudomonas fluorescens.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (CFC) by spread plate method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (25 ± 1) °C for (44 ± 4) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Growth.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material Pseudomonas fluorescens 100423
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.
  • 2 Quantitative process control with RM-AQUA

  • Growth. The characteristic reaction of  on CFC
    Characteristic reaction: Growth.

    Best Practice

    The multi-strain RM-AQUA contains Escherichia coli WDCM 00013, Pseudomonas fluorescens , Enterococcus faecalis WDCM 00087. This material can be used for several specific standards.The influence of interfering bacteria is also performed. It is easy to use by making one serie of dilution. This material is designed for water analysis

    1. Use a quantitative reference material (RM-AQUA) with a known number of Microorganisms.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (CFC) by spread plate method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (25 ± 1) °C for (44 ± 4) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Growth.

    Kind of reference material Biosisto product for this control method Control Strain WDCM number NCCB number
    Multi-strain reference material Enterococcus faecalis 00087 100299
    Escherichia coli 00013 100297
    Pseudomonas fluorescens 100423
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.
  • 3 Quantitative process control with RM-PA

  • Growth. The characteristic reaction of  on CFC
    Characteristic reaction: Growth.

    Best Practice

    The single strain RM-PA is used for the enumeration of Pseudomonas spp.

    1. Use a quantitative reference material (RM-PA) with a known number of Pseudomonas aeruginosa.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (CFC) by spread plate method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (25 ± 1) °C for (44 ± 4) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Growth.

    Kind of reference material Biosisto product for this control method Control Strain WDCM number
    Single-strain reference material Pseudomonas aeruginosa 00026
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.