ISO 8199 (2007) medium: Phosphate buffer solution

Media control Water quality 

Microorganisms and performance criteria for non-selective liquid media 'Phosphate buffer solution' used in Water quality  microbiology according to ISO 11133.

  • Quantitative control of Dilution

  • Best Practice Phosphate buffer solution

    1. Use a reference material or test suspension with a known number of Staphylococcus aureus.
    2. Inoculate a test portion (e.g. 9 mL) of the diluent with 1 mL of the test microorganism (Dilution liquids) suspension containing around 104 cfu/mL and mix;
    3. Immediately remove 0,1 mL of inoculated diluent and spread over the surface of a non-selective agar (reference medium) such as TSA (t0).
    4. Hold the inoculated diluent at ambient temperature for 45 min.
    5. Mix and then remove the same volume (0,1 mL) and plate again on the reference medium (t1).
    6. Incubate at ((20 °C – 25 °C)) for ((45 min – 1 h)).
    7. Count the colonies on the plates t0 and t1. The number of microorganisms at t1, shall be within ± 30 % of the initial count (t0).

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Staphylococcus aureus TSA (20 °C – 25 °C) for (45 min – 1 h) +/- 30 % colonies/ T0 (+/- 30 % of original count) No characteristic colonies
    Escherichia coli
    The best practice for Quantitative control of Dilution for media control of 'Phosphate buffer solution' is based on the data displayed in this table according ISO 11133.