ISO 11290 (all parts) (2017) medium: TSYEA

Media control Food chain

Microorganisms and performance criteria for non-selective liquid media 'TSYEA' used in Food chain microbiology according to ISO 11133.

  • Qualitative control of Productivity

  • Best Practice TSYEA

    Qualitative single tube method for performance testing of liquid media – pre-enrichment media

    1. Use a reference material or test suspension with a known number of Listeria monocytogenes 4b.
    2. Inoculate the media (TSYEA) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
    3. Incubate at (25 ± 1) °C for (21 ± 3) h.
    4. Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
    5. For this medium (TSYEA) and standard (ISO 11290 (all parts)) combination the criteria is: "Good Growth (2)."

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Listeria monocytogenes 4b (25 ± 1) °C for (21 ± 3) h Good Growth (2) Growth
    Listeria monocytogenes 1/2a
    The best practice for Qualitative control of Productivity for media control of 'TSYEA' is based on the data displayed in this table according ISO 11133.