Microorganisms and performance criteria for non-selective liquid media 'TSYEB' used in Food chain microbiology according to ISO 11133.
ISO 11290 (all parts) (2017) medium: TSYEB
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Qualitative control of Productivity
- Use a reference material or test suspension with a known number of Listeria monocytogenes 4b.
- Inoculate the media (TSYEB) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
- Incubate at (25 ± 1) °C for (21 ± 3) h.
- Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
- For this medium (TSYEB) and standard (ISO 11290 (all parts)) combination the criteria is: "Good Growth (2)."
Best Practice TSYEB
Qualitative single tube method for performance testing of liquid media – pre-enrichment media
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
---|---|---|---|---|---|
Listeria monocytogenes 4b | (25 ± 1) °C for (21 ± 3) h | Good Growth (2) | Growth | ||
Listeria monocytogenes 1/2a | |||||
The best practice for Qualitative control of Productivity for media control of 'TSYEB' is based on the data displayed in this table according ISO 11133. |