ISO 6888-3 (1999) medium: BHI

Media control Food and animal feeding stuffs

Microorganisms and performance criteria for non-selective liquid media 'BHI' used in Food and animal feeding stuffs microbiology according to ISO 11133.

  • Qualitative control of Productivity

  • Best Practice BHI

    Qualitative single tube method for performance testing of liquid media – pre-enrichment media

    1. Use a reference material or test suspension with a known number of Staphylococcus aureus.
    2. Inoculate the media (BHI) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
    3. Incubate at (37 ± 1) °C for (24 ± 2) h.
    4. Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
    5. For this medium (BHI) and standard (ISO 6888-3) combination the criteria is: "Turbidity (1–2)."

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Staphylococcus aureus (37 ± 1) °C for (24 ± 2) h Turbidity (1–2) Growth
    The best practice for Qualitative control of Productivity for media control of 'BHI' is based on the data displayed in this table according ISO 11133.