Microorganisms and performance criteria for testing non-selective media 'Columbia Blood Agar' for Enumeration of Campylobacter used in Food chain microbiology according to ISO 10272-2.
Qualitative control of Productivity
- Use a reference material or test suspension with a known number of Campylobacter jejuni and Campylobacter coli.
- The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
- Streak each test microorganism (Campylobacter jejuni and Campylobacter coli) on the media under test (Columbia Blood Agar) to obtain discrete colonies.
- Incubate at (41,5 ± 1) °C for 24h to 48 h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (Columbia Blood Agar) and standard (ISO 10272-2) combination the criteria is: "Good growth".
- The Characterictic reaction is: Growth.
Best Practice Columbia Blood Agar
Qualitative control of Productivity solid medium
|Control strain||WDCM number||Ref. media||Incubation||Criteria||Characteristic reaction|
|Campylobacter jejuni||Media batch blood agar already validated||(41,5 ± 1) °C for 24h to 48 h
|+ Campylobacter coli|
|The best practice for Qualitative control of Productivity for media control of 'Columbia Blood Agar' is based on the data displayed in this table according ISO 10272-2.|