ISO 10272-2 (2017) medium: Columbia Blood Agar

Media control Food chain

Microorganisms and performance criteria for testing non-selective media 'Columbia Blood Agar' for Enumeration of Campylobacter used in Food chain microbiology according to ISO 10272-2.

  • Qualitative control of Productivity

    • Growth. The characteristic reaction of on Columbia Blood Agar
    The characteristic reaction of Campylobacter jejuni on Columbia Blood Agar: Growth.

    Best Practice Columbia Blood Agar

    Qualitative control of Productivity solid medium

    1. Use a reference material or test suspension with a known number of Campylobacter jejuni and Campylobacter coli.
    2. The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
    3. Streak each test microorganism (Campylobacter jejuni and Campylobacter coli) on the media under test (Columbia Blood Agar) to obtain discrete colonies.
    4. Incubate at (41,5 ± 1) °C for 24h to 48 h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (Columbia Blood Agar) and standard (ISO 10272-2) combination the criteria is: "Good growth".
    7. The Characterictic reaction is: Growth.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Campylobacter jejuni
    or
    		Media batch blood agar already validated (41,5 ± 1) °C for 24h to 48 h
    microaerobic atmosphere    		 Good growth Growth
    + Campylobacter coli
    The best practice for Qualitative control of Productivity for media control of 'Columbia Blood Agar' is based on the data displayed in this table according ISO 10272-2.