ISO 6579-1 (2017) medium: Nutrient agar

Media control Food chain

Microorganisms and performance criteria for non-selective isolation media 'Nutrient agar' used in Food chain microbiology according to ISO 6579-1.

  • Qualitative control of Productivity

  • Best Practice Nutrient agar

    Qualitative control of Productivity solid medium

    1. Use a reference material or test suspension with a known number of Salmonella Typhimurium and Salmonella Enteritidis.
    2. The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
    3. Streak each test microorganism (Salmonella Typhimurium and Salmonella Enteritidis) on the media under test (Nutrient agar) to obtain discrete colonies.
    4. Incubate at 34 °C to 38 °C for 24 h ± 3 h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (Nutrient agar) and standard (ISO 6579-1) combination the criteria is: "Good growth".
    7. The Characterictic reaction is: Growth.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Salmonella Typhimurium 34 °C to 38 °C for 24 h ± 3 h Good growth Growth
    + Salmonella Enteritidis
    The best practice for Qualitative control of Productivity for media control of 'Nutrient agar' is based on the data displayed in this table according ISO 6579-1.