ISO 19250 (2013) medium: BPW

Media control Water quality 

Microorganisms and performance criteria for selective isolation media 'BPW' used in Water quality  microbiology according to ISO 11133.

  • Qualitative control of Productivity

    • Best Practice BPW

    Qualitative control of Selectivity of liquid medium, mixed culture

    1. Use a quantitative reference material or test suspension with a known number of Salmonella Typhimurium and Salmonella Enteritidis.
    2. Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
    3. Incubate at (36 ± 2) °C for (18 ± 2) h.
    4. Remove 10 µl and streak to a plate of specific medium for the target organism.
    5. Incubate at (36 ± 2) °C for (18 ± 2) h.
    6. Growth of target microorganisms should reach "Turbidity (1-2)" with a characteristic reaction of: Turbidity.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Salmonella Typhimurium
    		(36 ± 2) °C for (18 ± 2) h Turbidity (1-2) Turbidity
    + Salmonella Enteritidis
    The best practice for Qualitative control of Productivity for media control of 'BPW' is based on the data displayed in this table according ISO 11133.