ISO 21528-1 (2017) medium: BPW

Media control Food chain

Microorganisms and performance criteria for multipurpose media 'BPW' used in Food chain microbiology according to ISO 21528-1.

  • Qualitative control of Productivity

  • Best Practice BPW

    Qualitative single tube method for performance testing of liquid media – pre-enrichment media

    1. Use a reference material or test suspension with a known number of Escherichia coli.
    2. Inoculate the media (BPW) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
    3. Incubate at (37 ± 1) °C for (18 ± 2) h.
    4. Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
    5. For this medium (BPW) and standard (ISO 21528-1) combination the criteria is: "Turbidity (1–2)."

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Escherichia coli (37 ± 1) °C for (18 ± 2) h Turbidity (1–2) Turbidity
    Salmonella Typhimurium
    Salmonella Enteritidis
    The best practice for Qualitative control of Productivity for media control of 'BPW' is based on the data displayed in this table according ISO 21528-1.