ISO 6579-1 (2017) medium: BPW

Media control Food chain

Microorganisms and performance criteria for multipurpose media 'BPW' used in Food chain microbiology according to ISO 6579-1.

  • Qualitative control of Productivity

  • Best Practice BPW

    Qualitative single tube method for performance testing of liquid media – pre-enrichment media

    1. Use a reference material or test suspension with a known number of Salmonella Typhimurium and Salmonella Enteritidis.
    2. Inoculate the media (BPW) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
    3. Incubate at 34 °C to 38 °C for 18 h ± 2 h.
    4. Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
    5. For this medium (BPW) and standard (ISO 6579-1) combination the criteria is: "Turbidity (1–2)."

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Salmonella Typhimurium 34 °C to 38 °C for 18 h ± 2 h Turbidity (1–2) Turbidity (1–2)
    + Salmonella Enteritidis
    The best practice for Qualitative control of Productivity for media control of 'BPW' is based on the data displayed in this table according ISO 6579-1.