ISO 22964 (2017) medium: BPW

Media control Food chain

Microorganisms and performance criteria for non-selective liquid media 'BPW' used in Food chain microbiology according to ISO 22964.

  • Qualitative control of Productivity

    • Best Practice BPW

    Qualitative single tube method for performance testing of liquid media – pre-enrichment media

    1. Use a reference material or test suspension with a known number of Cronobacter sakazakii.
    2. Inoculate the media (BPW) with 0.1 mL of more than 100 cfu/mL and less then 1000 cfu/mL of microorganisms.
    3. Incubate at (18 ± 2) h for 34 °C to 38 °C.
    4. Asses the amount of growth as, 0 for no turbidity, 1 for slight turbidity or 2 for good turbidity.
    5. For this medium (BPW) and standard (ISO 22964) combination the criteria is: "Turbidity (1–2)."

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Cronobacter sakazakii
    		TSA (18 ± 2) h for 34 °C to 38 °C Turbidity (1–2) Turbidity
    Cronobacter muytjensii
    00213 opt
    The best practice for Qualitative control of Productivity for media control of 'BPW' is based on the data displayed in this table according ISO 22964.