Microorganisms and performance criteria for confirmation media 'CCI' used in Food chain microbiology according to ISO 22964.
ISO 22964 (2017) medium: CCI
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Qualitative control of Productivity
- Use a quantitative reference material or test suspension with a known number of Cronobacter sakazakii.
- Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
- Incubate at (41,5 ± 1) °C for (24 ± 2) h.
- Remove 10 µl and streak to a plate of specific medium for the target organism.
- Incubate at (41,5 ± 1) °C for (24 ± 2) h.
- Growth of target microorganisms should reach "Good growth (2)" with a characteristic reaction of: Small to mediumsized (1mm to 3mm) blue to bluegreen colonies.
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Qualitative control of Selectivity
- Use a reference material or test suspension with a known number of Staphylococcus aureus.
- Streak the test strain (Staphylococcus aureus) as a single line on the media under test (CCI).
- Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
- Incubate at (41,5 ± 1) °C for (24 ± 2) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (CCI) and standard (ISO 22964) combination the criteria is: "No growth.
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Qualitative control of Specificity
- Use a reference material or test suspension with a known number of Enterobacter cloacae.
- Streak the test controlstrain (Enterobacter cloacae) on the media under test (CCI) to obtain discrete colonies.
- Use a 1 µl loop in case of a concentration of at least 1000 cfu/mL.
- Incubate at (41,5 ± 1) °C for (24 ± 2) h.
- Asses the presence, absence and/or grade of expression of biochemical responses and colony sizes and morphology.
- For this medium (CCI) and standard (ISO 22964) combination the characteristic reaction is: Colonies do not have a green or bluegreen colour.

Best Practice CCI
Qualitative control of Selectivity of liquid medium, mixed culture
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
---|---|---|---|---|---|
Cronobacter sakazakii | (41,5 ± 1) °C for (24 ± 2) h | Good growth (2) | Small to mediumsized (1mm to 3mm) blue to bluegreen colonies | ||
Cronobacter muytjensii | |||||
The best practice for Qualitative control of Productivity for media control of 'CCI' is based on the data displayed in this table according ISO 22964. |
Best Practice CCI
Qualitative control of Selectivity solid medium
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Staphylococcus aureus | or | (41,5 ± 1) °C for (24 ± 2) h | No growth | No growth | |
The best practice for Qualitative control of Selectivity for media control of 'CCI' is based on the data displayed in this table according ISO 22964. |
Best Practice CCI
Qualitative control of Specificity solid medium
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Enterobacter cloacae | (41,5 ± 1) °C for (24 ± 2) h | Growth (1-2) | Colonies do not have a green or bluegreen colour | ||
The best practice for Qualitative control of Specificity for media control of 'CCI' is based on the data displayed in this table according ISO 22964. |