ISO 17995 (2005) medium: Bolton Preston

Media control Water quality 

Microorganisms and performance criteria for selective isolation media 'Bolton Preston' used in Water quality  microbiology according to ISO 11133.

  • 1 Qualitative control of Productivity

  • Best Practice Bolton Preston

    Qualitative control of Selectivity of liquid medium, mixed culture

    1. Use a quantitative reference material or test suspension with a known number of Campylobacter jejuni and Escherichia coli.
    2. Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
    3. Incubate at (37 ± 1) °C for (44 ± 4) h.
    4. Remove 10 µl and streak to a plate of specific medium for the target organism.
    5. Incubate at (37 ± 1) °C for (44 ± 4) h.
    6. Growth of target microorganisms should reach "> 10 colonies on mCCDA" with a characteristic reaction of: Small, flat or convex colonies with a glossy surface.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Campylobacter jejuni or (37 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    > 10 colonies on mCCDA Small, flat or convex colonies with a glossy surface
    + Escherichia coli or
    Proteus mirabilis
    The best practice for Qualitative control of Productivity for media control of 'Bolton Preston' is based on the data displayed in this table according ISO 11133.
  • 2 Qualitative control of Productivity

  • Best Practice Bolton Preston

    Qualitative control of Selectivity of liquid medium, mixed culture

    1. Use a quantitative reference material or test suspension with a known number of Campylobacter coli and Escherichia coli and Proteus mirabilis.
    2. Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
    3. Incubate at (37 ± 1) °C for (44 ± 4) h.
    4. Remove 10 µl and streak to a plate of specific medium for the target organism.
    5. Incubate at (37 ± 1) °C for (44 ± 4) h.
    6. Growth of target microorganisms should reach "> 10 colonies on mCCDA" with a Characteristic reaction of: Small, flat or convex colonies with a glossy surface.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Campylobacter coli (37 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    > 10 colonies on mCCDA Small, flat or convex colonies with a glossy surface
    + Escherichia coli or
    + Proteus mirabilis
    The best practice for Qualitative control of Productivity for media control of 'Bolton Preston' is based on the data displayed in this table according ISO 11133.
  • Qualitative control of Selectivity

  • Best Practice Bolton Preston

    Qualitative control of Selectivity of liquid medium, single culture

    1. Use a quantitative reference material or test suspension with a known number of Escherichia coli and Proteus mirabilis.
    2. Inoculate one tube of test medium with >103 cells of non-target organisms.
    3. Incubate at (37 ± 1) °C for (44 ± 4) h.
    4. Remove 10 µl and streak to a plate of reference medium (TSA).
    5. Incubate at (37 ± 1) °C for (44 ± 4) h.
    6. Growth of non-target microorganisms should comply with the criteria: "Total inhibition on TSA (0)".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli or TSA (37 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    Total inhibition on TSA (0) No growth
    + Proteus mirabilis
    The best practice for Qualitative control of Selectivity for media control of 'Bolton Preston' is based on the data displayed in this table according ISO 11133.