Microorganisms and performance criteria for selective enrichment media 'TSPB' used in Food and animal feeding stuffs microbiology according to ISO 11133.
ISO 21871 (2006) medium: TSPB
-
Qualitative control of Productivity
- Use a quantitative reference material or test suspension with a known number of Bacillus cereus.
- Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
- Incubate at (30 ± 1) °C for (48 ± 4) h.
- Remove 10 µl and streak to a plate of specific medium for the target organism.
- Incubate at (30 ± 1) °C for (48 ± 4) h.
- Growth of target microorganisms should reach "> 10 colonies on PEMBA or MYP" with a characteristic reaction of: Characteristic colonies according to each medium (see ISO 21871).
-
Qualitative control of Selectivity
- Use a quantitative reference material or test suspension with a known number of Escherichia coli.
- Inoculate one tube of test medium with >103 cells of non-target organisms.
- Incubate at (30 ± 1) °C for (48 ± 4) h.
- Remove 10 µl and streak to a plate of reference medium (TSA).
- Incubate at (30 ± 1) °C for (48 ± 4) h.
- Growth of non-target microorganisms should comply with the criteria: "Total inhibition (0) on TSA".
Best Practice TSPB
Qualitative control of Selectivity of liquid medium, mixed culture
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
---|---|---|---|---|---|
Bacillus cereus | (30 ± 1) °C for (48 ± 4) h | > 10 colonies on PEMBA or MYP | Characteristic colonies according to each medium (see ISO 21871) | ||
The best practice for Qualitative control of Productivity for media control of 'TSPB' is based on the data displayed in this table according ISO 11133. |
Best Practice TSPB
Qualitative control of Selectivity of liquid medium, single culture
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Escherichia coli | or | TSA | (30 ± 1) °C for (48 ± 4) h | Total inhibition (0) on TSA | No growth |
The best practice for Qualitative control of Selectivity for media control of 'TSPB' is based on the data displayed in this table according ISO 11133. |