ISO 21871 (2006) medium: TSPB

Media control Food and animal feeding stuffs

Microorganisms and performance criteria for selective enrichment media 'TSPB' used in Food and animal feeding stuffs microbiology according to ISO 11133.

  • Qualitative control of Productivity

    • Best Practice TSPB

    Qualitative control of Selectivity of liquid medium, mixed culture

    1. Use a quantitative reference material or test suspension with a known number of Bacillus cereus.
    2. Inoculate one tube of test medium with < 100 cells of target microorganisms and with >103 cells of non-target organisms.
    3. Incubate at (30 ± 1) °C for (48 ± 4) h.
    4. Remove 10 µl and streak to a plate of specific medium for the target organism.
    5. Incubate at (30 ± 1) °C for (48 ± 4) h.
    6. Growth of target microorganisms should reach "> 10 colonies on PEMBA or MYP" with a characteristic reaction of: Characteristic colonies according to each medium (see ISO 21871).

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Bacillus cereus
    		(30 ± 1) °C for (48 ± 4) h > 10 colonies on PEMBA or MYP Characteristic colonies according to each medium (see ISO 21871)
    The best practice for Qualitative control of Productivity for media control of 'TSPB' is based on the data displayed in this table according ISO 11133.

  • Qualitative control of Selectivity

    • Best Practice TSPB

    Qualitative control of Selectivity of liquid medium, single culture

    1. Use a quantitative reference material or test suspension with a known number of Escherichia coli.
    2. Inoculate one tube of test medium with >103 cells of non-target organisms.
    3. Incubate at (30 ± 1) °C for (48 ± 4) h.
    4. Remove 10 µl and streak to a plate of reference medium (TSA).
    5. Incubate at (30 ± 1) °C for (48 ± 4) h.
    6. Growth of non-target microorganisms should comply with the criteria: "Total inhibition (0) on TSA".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli
    or
    		TSA (30 ± 1) °C for (48 ± 4) h Total inhibition (0) on TSA No growth
    The best practice for Qualitative control of Selectivity for media control of 'TSPB' is based on the data displayed in this table according ISO 11133.