Microorganisms and performance criteria for selective enrichment media 'MSRV agar' used in Food chain microbiology according to ISO 6579-1.
ISO 6579-1 (2017) medium: MSRV agar
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Qualitative control of Productivity
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1 Qualitative control of Selectivity
- Use a quantitative reference material or test suspension with a known number of Escherichia coli.
- Inoculate one tube of test medium with >103 cells of non-target organisms.
- Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
- Remove 10 µl and streak to a plate of reference medium (TSA).
- Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
- Growth of non-target microorganisms should comply with the criteria: "Possible growth at the place of the inoculated drop(s) without a turbid zone".
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2 Qualitative control of Selectivity
- Use a quantitative reference material or test suspension with a known number of Enterococcus faecalis.
- Inoculate one tube of test medium with >103 cells of non-target organisms.
- Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
- Remove 10 µl and streak to a plate of reference medium (TSA).
- Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
- Growth of non-target microorganisms should comply with the criteria: "No Growth".
Best Practice MSRV agar
Strain free of choice; one of the strains has to be used as a minimum
No further Best Practice available. For the correct method of testing see ISO 11133.
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
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Salmonella Typhimurium | 41,5 °C ± 1 °C for 2x(24 h ± 3 h) | Grey-white, turbid zone extending out from inoculated drop(s). After 24 h to 48 h, the turbid zone(s) will be (almost) fully migrated over the plate. | Possible extra: characteristic colonies after subculturing on XLD agar | ||
Salmonella Enteritidis | or | ||||
The best practice for Qualitative control of Productivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1. |
Best Practice MSRV agar
Qualitative control of Selectivity of liquid medium, single culture
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
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Escherichia coli | or | TSA | 41,5 °C ± 1 °C for 2x(24 h ± 3 h) | Possible growth at the place of the inoculated drop(s) without a turbid zone | Possible growth at the place of the inoculated drop(s) without a turbid zone |
The best practice for Qualitative control of Selectivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1. |
Best Practice MSRV agar
Qualitative control of Selectivity of liquid medium, single culture
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Enterococcus faecalis | or | TSA | 41,5 °C ± 1 °C for 2x(24 h ± 3 h) | No Growth | No Growth |
The best practice for Qualitative control of Selectivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1. |