ISO 6579-1 (2017) medium: MSRV agar

Media control Food chain

Microorganisms and performance criteria for selective enrichment media 'MSRV agar' used in Food chain microbiology according to ISO 6579-1.

  • Qualitative control of Productivity

    • Best Practice MSRV agar

    Strain free of choice; one of the strains has to be used as a minimum

    No further Best Practice available. For the correct method of testing see ISO 11133.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Salmonella Typhimurium
    		41,5 °C ± 1 °C for 2x(24 h ± 3 h) Grey-white, turbid zone extending out from inoculated drop(s). After 24 h to 48 h, the turbid zone(s) will be (almost) fully migrated over the plate. Possible extra: characteristic colonies after subculturing on XLD agar
    Salmonella Enteritidis or
    The best practice for Qualitative control of Productivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1.

  • 1 Qualitative control of Selectivity

    • Best Practice MSRV agar

    Qualitative control of Selectivity of liquid medium, single culture

    1. Use a quantitative reference material or test suspension with a known number of Escherichia coli.
    2. Inoculate one tube of test medium with >103 cells of non-target organisms.
    3. Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
    4. Remove 10 µl and streak to a plate of reference medium (TSA).
    5. Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
    6. Growth of non-target microorganisms should comply with the criteria: "Possible growth at the place of the inoculated drop(s) without a turbid zone".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli
    or
    		TSA 41,5 °C ± 1 °C for 2x(24 h ± 3 h) Possible growth at the place of the inoculated drop(s) without a turbid zone Possible growth at the place of the inoculated drop(s) without a turbid zone
    The best practice for Qualitative control of Selectivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1.

  • 2 Qualitative control of Selectivity

    • Best Practice MSRV agar

    Qualitative control of Selectivity of liquid medium, single culture

    1. Use a quantitative reference material or test suspension with a known number of Enterococcus faecalis.
    2. Inoculate one tube of test medium with >103 cells of non-target organisms.
    3. Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
    4. Remove 10 µl and streak to a plate of reference medium (TSA).
    5. Incubate at 41,5 °C ± 1 °C for 2x(24 h ± 3 h).
    6. Growth of non-target microorganisms should comply with the criteria: "No Growth".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Enterococcus faecalis
    or
    		TSA 41,5 °C ± 1 °C for 2x(24 h ± 3 h) No Growth No Growth
    The best practice for Qualitative control of Selectivity for media control of 'MSRV agar' is based on the data displayed in this table according ISO 6579-1.