ISO 9308-2 (2014) medium: Colilert-18

Media control Water quality 

Microorganisms and performance criteria for selective isolation media 'Colilert-18' used in Water quality  microbiology according to ISO 11133.

  • 1 Quantitative control of Productivity

  • Best Practice Colilert-18

    Please refer to 11133 for best practices

    No further Best Practice available. For the correct method of testing see ISO 11133.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli TSA (36 ± 2) °C for (20 ± 2)h PR ≥ 0,5 Yellow colour and fluorescence for E. coli
    The best practice for Quantitative control of Productivity for media control of 'Colilert-18' is based on the data displayed in this table according ISO 11133.
  • 2 Quantitative control of Productivity

  • Best Practice Colilert-18

    Please refer to 11133 for best practices

    No further Best Practice available. For the correct method of testing see ISO 11133.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Klebsiella variicola TSA (36 ± 2) °C for (20 ± 2)h PR ≥ 0,5 Yellow colour equal or greater than the comparator for coliform bacteria
    The best practice for Quantitative control of Productivity for media control of 'Colilert-18' is based on the data displayed in this table according ISO 11133.
  • 3 Quantitative control of Productivity

  • Best Practice Colilert-18

    Please refer to 11133 for best practices

    No further Best Practice available. For the correct method of testing see ISO 11133.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli previously validated batch Colilert (36 ± 2) °C for (20 ± 2)h PR ≥ 0,7 Yellow colour and fluorescence for E. coli
    The best practice for Quantitative control of Productivity for media control of 'Colilert-18' is based on the data displayed in this table according ISO 11133.
  • 4 Quantitative control of Productivity

  • Best Practice Colilert-18

    Please refer to 11133 for best practices

    No further Best Practice available. For the correct method of testing see ISO 11133.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Klebsiella variicola previously validated batch Colilert (36 ± 2) °C for (20 ± 2)h PR ≥ 0,7 Yellow colour equal or greater than the comparator for coliform bacteria
    The best practice for Quantitative control of Productivity for media control of 'Colilert-18' is based on the data displayed in this table according ISO 11133.
  • Qualitative control of Selectivity

  • Best Practice Colilert-18

    Please refer to 11133 for best practices

    Qualitative control of Selectivity of liquid medium, single culture

    1. Use a quantitative reference material or test suspension with a known number of Pseudomonas aeruginosa.
    2. Inoculate one tube of test medium with >103 cells of non-target organisms.
    3. Incubate at (36 ± 2) °C for (20 ± 2)h.
    4. Remove 10 µl and streak to a plate of reference medium .
    5. Incubate at (36 ± 2) °C for (20 ± 2)h.
    6. Growth of non-target microorganisms should comply with the criteria: "Total inhibition (0)".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Pseudomonas aeruginosa or (36 ± 2) °C for (20 ± 2)h Total inhibition (0) Less yellow than the comparator
    The best practice for Qualitative control of Selectivity for media control of 'Colilert-18' is based on the data displayed in this table according ISO 11133.