ISO 10272-1 (2017) medium: mCCDA

Media control Food chain

Microorganisms and performance criteria for selective isolation media 'mCCDA' used in Food chain microbiology according to ISO 10272-1.

  • Qualitative control of Productivity

  • Best Practice mCCDA

    Qualitative control of Productivity solid medium

    1. Use a reference material or test suspension with a known number of Campylobacter jejuni.
    2. The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
    3. Streak the test microorganism (Campylobacter jejuni) on the media under test (mCCDA) to obtain discrete colonies.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 10272-1) combination the criteria is: "Good growth (2)".
    7. The Characterictic reaction is: Greyish, flat and moist colonies, sometimes with metallic sheen.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Campylobacter jejuni or (41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    Good growth (2) Greyish, flat and moist colonies, sometimes with metallic sheen
    Campylobacter coli
    The best practice for Qualitative control of Productivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 10272-1.
  • 1 Qualitative control of Selectivity

  • Best Practice mCCDA

    Qualitative control of Selectivity solid medium

    1. Use a reference material or test suspension with a known number of Escherichia coli.
    2. Streak the test strain (Escherichia coli) as a single line on the media under test (mCCDA).
    3. Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 10272-1) combination the criteria is: "Total or partial inhibition (0-1).

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli or (41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    Total or partial inhibition (0-1) No characteristic colonies
    The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 10272-1.
  • 2 Qualitative control of Selectivity opt

  • Best Practice mCCDA

    Qualitative control of Selectivity solid medium

    1. Use a reference material or test suspension with a known number of Staphylococcus aureus.
    2. Streak the test strain (Staphylococcus aureus) as a single line on the media under test (mCCDA).
    3. Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 10272-1) combination the criteria is: "Total inhibition (0)".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Staphylococcus aureus (41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere
    Total inhibition (0) No growth
    The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 10272-1.