ISO 17995 (2005) medium: mCCDA

Media control Water quality 

Microorganisms and performance criteria for selective isolation media 'mCCDA' used in Water quality  microbiology according to ISO 11133.

  • Qualitative control of Productivity

    • Best Practice mCCDA

    Qualitative control of Productivity solid medium

    1. Use a reference material or test suspension with a known number of Campylobacter jejuni.
    2. The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
    3. Streak the test microorganism (Campylobacter jejuni) on the media under test (mCCDA) to obtain discrete colonies.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Good growth (2)".
    7. The Characterictic reaction is: Small, flat or convex colonies with a glossy surface.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Campylobacter jejuni
    00005 opt
    		 (41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere    		 Good growth (2) Small, flat or convex colonies with a glossy surface
    Campylobacter coli
    00004 opt
    The best practice for Qualitative control of Productivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133.

  • 1 Qualitative control of Selectivity man

    • Best Practice mCCDA

    Qualitative control of Selectivity solid medium

    1. Use a reference material or test suspension with a known number of Escherichia coli.
    2. Streak the test strain (Escherichia coli) as a single line on the media under test (mCCDA).
    3. Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Total or partial inhibition (0−1).

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli
    or
    or
    or
    		(41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere    		 Total or partial inhibition (0−1) No characteristic colonies
    The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133.

  • 2 Qualitative control of Selectivity man

    • Best Practice mCCDA

    Qualitative control of Selectivity solid medium

    1. Use a reference material or test suspension with a known number of Staphylococcus aureus.
    2. Streak the test strain (Staphylococcus aureus) as a single line on the media under test (mCCDA).
    3. Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
    4. Incubate at (41,5 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Total inhibition (0)".

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Staphylococcus aureus
    or
    		(41,5 ± 1) °C for (44 ± 4) h
    microaerobic atmosphere    		 Total inhibition (0) No growth
    The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133.