Microorganisms and performance criteria for selective isolation media 'mCCDA' used in Water quality microbiology according to ISO 11133.
ISO 17995 (2005) medium: mCCDA
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Qualitative control of Productivity
- Use a reference material or test suspension with a known number of Campylobacter jejuni.
- The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
- Streak the test microorganism (Campylobacter jejuni) on the media under test (mCCDA) to obtain discrete colonies.
- Incubate at (41,5 ± 1) °C for (44 ± 4) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Good growth (2)".
- The Characterictic reaction is: Small, flat or convex colonies with a glossy surface.
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1 Qualitative control of Selectivity man
- Use a reference material or test suspension with a known number of Escherichia coli.
- Streak the test strain (Escherichia coli) as a single line on the media under test (mCCDA).
- Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
- Incubate at (41,5 ± 1) °C for (44 ± 4) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Total or partial inhibition (0−1).
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2 Qualitative control of Selectivity man
- Use a reference material or test suspension with a known number of Staphylococcus aureus.
- Streak the test strain (Staphylococcus aureus) as a single line on the media under test (mCCDA).
- Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
- Incubate at (41,5 ± 1) °C for (44 ± 4) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (mCCDA) and standard (ISO 17995) combination the criteria is: "Total inhibition (0)".
Best Practice mCCDA
Qualitative control of Productivity solid medium
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
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Campylobacter jejuni | (41,5 ± 1) °C for (44 ± 4) h microaerobic atmosphere |
Good growth (2) | Small, flat or convex colonies with a glossy surface | ||
Campylobacter coli | |||||
The best practice for Qualitative control of Productivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133. |
Best Practice mCCDA
Qualitative control of Selectivity solid medium
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Escherichia coli | or or or | (41,5 ± 1) °C for (44 ± 4) h microaerobic atmosphere |
Total or partial inhibition (0−1) | No characteristic colonies | |
The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133. |
Best Practice mCCDA
Qualitative control of Selectivity solid medium
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Staphylococcus aureus | or | (41,5 ± 1) °C for (44 ± 4) h microaerobic atmosphere |
Total inhibition (0) | No growth | |
The best practice for Qualitative control of Selectivity for media control of 'mCCDA' is based on the data displayed in this table according ISO 11133. |