ISO 21871 (2006) medium: PEMBA

Media control Food and animal feeding stuffs

Microorganisms and performance criteria for selective isolation media 'PEMBA' used in Food and animal feeding stuffs microbiology according to ISO 11133.

  • Qualitative control of Productivity

  • Best Practice PEMBA

    Qualitative control of Productivity solid medium

    1. Use a reference material or test suspension with a known number of Bacillus cereus.
    2. The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
    3. Streak the test microorganism (Bacillus cereus) on the media under test (PEMBA) to obtain discrete colonies.
    4. Incubate at (37 ± 1) °C for (21 ± 3) h to (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (PEMBA) and standard (ISO 21871) combination the criteria is: "Good growth (2)".
    7. The Characterictic reaction is: Turquoise-blue colonies with precipitation halo.

    Control strain WDCM number Incubation Criteria Characteristic reaction
    Bacillus cereus (37 ± 1) °C for (21 ± 3) h to (44 ± 4) h Good growth (2) Turquoise-blue colonies with precipitation halo
    The best practice for Qualitative control of Productivity for media control of 'PEMBA' is based on the data displayed in this table according ISO 11133.
  • Qualitative control of Selectivity

  • Best Practice PEMBA

    Qualitative control of Selectivity solid medium

    1. Use a reference material or test suspension with a known number of Escherichia coli.
    2. Streak the test strain (Escherichia coli) as a single line on the media under test (PEMBA).
    3. Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
    4. Incubate at (37 ± 1) °C for (44 ± 4) h.
    5. Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
    6. For this medium (PEMBA) and standard (ISO 21871) combination the criteria is: "Total inhibition (0).

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Escherichia coli or (37 ± 1) °C for (44 ± 4) h Total inhibition (0) No growth
    The best practice for Qualitative control of Selectivity for media control of 'PEMBA' is based on the data displayed in this table according ISO 11133.
  • Qualitative control of Specificity

  • Best Practice PEMBA

    Qualitative control of Specificity solid medium

    1. Use a reference material or test suspension with a known number of Bacillus subtilis subsp. spizizenii.
    2. Streak the test controlstrain (Bacillus subtilis subsp. spizizenii) on the media under test (PEMBA) to obtain discrete colonies.
    3. Use a 1 µl loop in case of a concentration of at least 1000 cfu/mL.
    4. Incubate at (37 ± 1) °C for (44 ± 4) h.
    5. Asses the presence, absence and/or grade of expression of biochemical responses and colony sizes and morphology.
    6. For this medium (PEMBA) and standard (ISO 21871) combination the characteristic reaction is: White colonies without precipitation halo.

    Control strain WDCM number Ref. media Incubation Criteria Characteristic reaction
    Bacillus subtilis subsp. spizizenii (37 ± 1) °C for (44 ± 4) h Characteristic growth White colonies without precipitation halo
    The best practice for Qualitative control of Specificity for media control of 'PEMBA' is based on the data displayed in this table according ISO 11133.