Microorganisms and performance criteria for selective isolation media 'TCBS' used in Food chain microbiology according to ISO 21872-1.
ISO 21872-1 (2017) medium: TCBS
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Qualitative control of Productivity
- Use a reference material or test suspension with a known number of Vibrio parahaemolyticus and Vibrio furnissii.
- The volume used for testing should contain 103 to 104 cfu for qualitative tests of plate media.
- Streak each test microorganism (Vibrio parahaemolyticus and Vibrio furnissii) on the media under test (TCBS) to obtain discrete colonies.
- Incubate at (37 ± 1) °C for (24 ± 3) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (TCBS) and standard (ISO 21872-1) combination the criteria is: "Good growth (2)".
- The Characterictic reaction is: Green colonies (sucrose negative).
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Qualitative control of Selectivity
- Use a reference material or test suspension with a known number of Escherichia coli.
- Streak the test strain (Escherichia coli) as a single line on the media under test (TCBS).
- Use a 1 µl loop in case of a concentration of at least 10000 cfu or a 10 µl loop in case of a concentration between 1000 and 10000 cfu.
- Incubate at (37 ± 1) °C for (24 ± 3) h.
- Asses the amount of growth as, 0 for no growth, 1 for weak growth or 2 for good growth.
- For this medium (TCBS) and standard (ISO 21872-1) combination the criteria is: "Total inhibition (0).

Best Practice TCBS
Qualitative control of Productivity solid medium
Control strain | WDCM number | Incubation | Criteria | Characteristic reaction | |
---|---|---|---|---|---|
Vibrio parahaemolyticus | (37 ± 1) °C for (24 ± 3) h | Good growth (2) | Green colonies (sucrose negative) | ||
+ Vibrio furnissii | |||||
The best practice for Qualitative control of Productivity for media control of 'TCBS' is based on the data displayed in this table according ISO 21872-1. |
Best Practice TCBS
Qualitative control of Selectivity solid medium
Control strain | WDCM number | Ref. media | Incubation | Criteria | Characteristic reaction |
---|---|---|---|---|---|
Escherichia coli | or or | (37 ± 1) °C for (24 ± 3) h | Total inhibition (0) | No growth | |
The best practice for Qualitative control of Selectivity for media control of 'TCBS' is based on the data displayed in this table according ISO 21872-1. |