Cronobacter sakazakii is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. A part of the analysis is a streak on CCI agar. Therefore, the quality of the CCI medium is crucial to the analysis. Cronobacter sakazakii should show blue to blue-green …

Many lactobacillus species have been reclassified. This will majorly impact the market because many nutritional and probiotic supplements contain Lactobacilli. New genetic sequencing methods have led to classifying the current lactobacillus into several new groups. For Biosisto, the strain Lactobacillus plantarum in CRM-LP and CRM-BLES changes the name in Lactiplantibacillus plantarum. The new name tells …

The property value on the Certificate of Analysis is, in most cases, performed according to a standard like ISO or NEN. Sometimes is no standard available, and a total count of micro-organisms is displayed using a non-selective media. This is the case with quality products and CRM with low concentrations, whereby besides the standard method …

Microorganisms can be divided and analyzed into the following categories and standards:   Category Treatment Min.°C Optimum.°C Max. °C Standard Psychrophilic cell – -15 10 20 – Psychrotrophic cell – -5 25 35 ISO 6222,  ISO 21527-1,  ISO 21527-2 Mesophilic cell Mesophilic spore – 10 min 80°C 10 35 45 ISO 4833-1,  4833-2 NEN 6813 …

This WDCM (World Data Centre of Microorganisms) catalog was produced to enable broader and easier access to the reference strains. This collection is affiliated with the World Federation for Culture Collections (WFCC), a collection of various national culture collections. It fulfills a need expressed by these bodies for a unique system of identifiers for strains recommended …

Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

Information and articles about Listeria spp. : Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) NVWA – Informatieblad 85 Interpretatiedocument microbiologische criteria (NL) RvA – Protocol (SAP) voor accreditatie van challengetesten Listeria monocytogenes (NL) VMT artikel – Het uitroeien van persisterende Listeria monocytogenes-stammen (NL) VMT …

CRM-BRETT contains alive Brettanomyces bruxellensis now available! Analyses for Brettanomyces bruxellensis are very important for wineries. The presence of Brettanomyces bruxellensis indicates spoilage of the wine. Analyzing Brettanomyces bruxellensis by flow cytometry is a quick way to determine if Brettanomyces bruxellensis is present in the wine. Laboratoria investigating the wine should be reported reliable results. False-positive …

Congratulations on using the Biosisto Chart! If not ask for an account at info@biosisto.com You will experience that this is an easy online tool for registering the CRM results. With the Quick Start Guide in 4 steps v2, we help you to start up the BiosistoChart very easily quickly. This Guide described the basic functions. …

ISO 19344 Starter cultures, probiotics, and fermented products – Quantification of lactic acid bacteria by flow cytometry. For different applications Biosisto measures alive and dead cells according to ISO 19344 protocol B using SYTO® 24 (green fluorescent cell-permeant nucleic acid stain) and PI (Propidium iodide is a red-fluorescent nuclear and chromosome counterstain. According to ISO …

Brettanomyces bruxellensis is a yeast that is part of the Ascomycota phylum and plays a key role in the production of several Belgian beers, as well as provides the wine with certain aroma’s. Brettanomyces bruxellensis is also known as Dekkara bruxellensis, where the name that is used depends on the stage in its life cycle. …

The CRM-BRETT is designed for internal quality control for the analysis of Brettanomyces bruxellensis in wine using the Cyflow BrettCount kit from Sysmex. The reference material contains alive Brettanomyces bruxellensis. With this Certified Reference Material, the entire analysis is checked for correctness, such as the work-up, staining, and the final analysis with the flow cytometry. …

  Geobacillus stearothermophilus under the microscope. Cell staining with safranine gives red cells Spore staining with malachite green gives green spores …

The source of the Biosisto strains is the NCCB*1 collection or the CCUG*2 collection. In addition, Biosisto will deposit the strain into the NCCB collection if the strain comes from a customer. You can find the strains we use at the culture bank websites, see related links below. The choice of strain(s) in Biosisto CRM products depends …

Listeria monocytogenes challenge testing is now worthy of accreditation. From now on, the Council for Accreditation (RvA) considers challenge tests for Listeria monocytogenes within microbiology to be worthy of accreditation. This gives test laboratories the opportunity to submit an accreditation or extension request for this. Biosisto has 11 Listeria Certified Reference Materials available to help …

Biosisto has for the process control of ISO 4833 (1 and 2) four different Certified Reference Materials available each with their own characteristics. What you choose depends on what the requirement of your laboratory is. The differences are schematically shown in the table below. Single or multi-strain CRM Accreditated Metrological Traceability of Media Performance testing …

Listeria monocytogenes is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. The quality of the ALOA medium used is a crucial part of the analysis. Research shows that there is a difference in electivity between suppliers. The color of the colony …

CRM-AQUA contains 3 different strains each with their growth optimums. The certificate of Analysis shows two total count method property values namely for 36°C and 22°C incubation. There is a difference in concentration is this two incubation temperatures that is caused by Pseudomonas fluorescens. This strain is not able to grow at 36°C. At 22°C …

Using Certified Reference Materials (CRM) daily gives a quick and easy insight into the used methods’ performance. Especially for water analyses, are products suitable for filtration and other water methods available. Use Certified Reference Materials (CRM) in Quality-Control Programs for: • Daily process control • Performance testing of culture media according to ISO 11133 • …

Two DRBC quality issues you should know. This article may help you troubleshoot quality issues with the DRBC medium. DRBC medium is described in ISO 21527-1 for the isolation and enumeration of yeast and moulds. DRBC is the abbreviation of Dichloran Rose-Bengal Chloramphenicol Agar. Rose-bengal photo-oxidizes to form toxic compounds. Store DRBC plates in the …

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Without matrix With matrix …

CRM-LPN.BH analysis according to ISO 11731 A property value is given on the Certificate of analysis for 5 different analyses using the following methods: Without filtration (direct plating) 0,1 ml RM is diluted in 4,9 ml PFZ 0,1 ml is plated on the media With filtration, without treatment (WT) Bring 50 ml sterile water on …

Many aspects play a key role in the analysis of ISO 11731, such as filters, washing the filter and media. To verify all aspects of the analysis, Biosisto created new products that are specially designed for this analysis. The products CRM-LPN.BH, CRM-LAN.00106BL and CRM-LPN.00107BH can be used for media control, quality control, learning purposes, verification …

There is a lot of information on the Certificat of Analysis. What the information tells you is explanted of the Certificate of Analysis over here …