The source of the Biosisto strains is the NCCB*1 collection or the CCUG*2 collection.
In addition, Biosisto will deposit the strain into the NCCB collection if the strain comes from a customer.
You can find the strains we use at the culture bank websites, see related links below.
The choice of strain(s) in Biosisto CRM products depends on the purpose of the quality control. For instance:
– Strains that fully comply with ISO 11133, displayed in a WDCM number.
These strains are meant for media performance testing and, in most cases, also for Internal Quality Control (IQC)
– According to the analyses Standard (ISO), Strains must be used, displayed in a WDCM number.
If the analysis standard is more recent than the ISO 11133, the suggested strains in the analysis standard must be used.
– Wild strains suitable for Internal Quality Control (IQC).
These strains are often more robust than the strains mentioned in ISO 11133. For instance, the wild strain Legionella pneumophila can survive as heat treatment, as mentioned in the ISO 11731. While the Legionella pneumophila WDCM 00107 mentioned in the ISO 11133 cannot survive the heat treatment and is therefore not suitable for IQC.
– A multi-strain CRM contains more than 1 strain. The reason for a multi-strain can be different. For example, it more closely approximates the reality of analysis samples or because multiple IQC analyzes can be done with one CRM.
– Strain(s) on special requests of a customer.
WDCM is set up by the WFCC*4. This is a network of microbial resource centers of various types of microbes. The NCCB and the CCUG collection are part of the WFCC. At the website WDCM Reference Strain Catalogue, you can find the equivalent of the WDCM number in different strain collections. In addition, the origin of the strain can be found in these strain collections.
*1 NCCB – Netherlands Culture Collection of Bacteria
*2 CCUG – Culture Collection University of Gothenburg
*3 WDCM – World Data Centre of Microorganisms
*4 WFCC – World Federation for Culture Collections