TSC (SC) media supplier comparison

Posted - Author Annemarie
For information about TSC (SC)

Sometimes it is useful to know to see that media from supplier to supplier can give different results in appearance and productivity.
For example, TSC (SC) medium was used to analyze Clostridium perfringens.

CRM-CP batch 04P-2205 was analyzed simultaneously on TSC (SC) media of 3 different suppliers according to ISO 7937.
The appearance of the TSC (SC) agar was different.
On one media, the Clostridium perfringens is more enthusiastic with the black coloring than on the other. See the examples below.

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Horizontal method for the enumeration of Clostridium perfringens – Colony-count technique …

ISO 7937
Standards

If you do not find black colonies, or fewer colonies,  on the agar plate by the analyses of Clostridium perfringens or Sulfite-reducing bacteria, it can have several causes. Checking the below items in your laboratory to solve the issue. Is the media fit for purpose? Did you perform a media performance test according to ISO …

Why do I find no black colony during the Clostridium analysis?
FAQ

Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

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