ISO 7899-2 (2000) method: Intestinal enterococci

Process control Water quality 

Detection and enumeration of Intestinal enterococci - Part 2: Membrane filtration method

  • 1 Quantitative process control with RM-EFA.00009BL

  • Red-maroon-pink colonies. The characteristic reaction of  on Slanetz and Bartley
    Characteristic reaction: Red-maroon-pink colonies.

    Best Practice

    The single strain RM-EFA.00009BL is used for the enumeration of Intestinal enterococci

    1. Use a quantitative reference material (RM-EFA.00009BL) with a known number of Enterococcus faecalis.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (Slanetz and Bartley) by membrane filtration method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (36 ± 2) °C for (44 ± 4) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Red-maroon-pink colonies.

    Kind of reference material Biosisto product for this control method Control Strain WDCM number
    Single-strain reference material Enterococcus faecalis 00009
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.
  • 2 Quantitative process control with RM-EFAEC

  • Best Practice

    The single strain RM-EFAEC is used for the enumeration of Intestinal enterococci

    1. Use a quantitative reference material (RM-EFAEC) with a known number of Enterococcus faecium.
    2. Defrost the material at room temperature for 30 minutes.
    3. Mix well before use and dilute the material so countable numbers are inoculated on the media (Slanetz and Bartley) by membrane filtration method.
    4. Best results are obtained when a practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate is used.
    5. Incubate at (36 ± 2) °C for (44 ± 4) h.
    6. Count the colony forming units and register in a control chart.
    7. The characteristic reaction is: Red-maroon-pink colonies.

    Kind of reference material Biosisto product for this control method Control Strain NCCB number
    Single-strain reference material Enterococcus faecium 100615
    The Biosisto reference material in this table is used in the best practice for Quantitative process control according ISO 17025.