BiosistoStandard

BiosistoStandard has the answer to your practical microbiological quality issues.

BiosistoStandard is a guide that helps you find quick the necessary Certified Reference Materials for your quality program. The website gives you also a lot of practical information, photos, tips & tricks that can help you optimize the quality of the analysis.

The recommended Certified Reference Materials for performance testing suggestions are a Biosisto interpretation of the ISO 11133 and the corresponding analysis standard. The suggested strain(s) has to be used as a minimum.

Do you have helpful information that other labs should know? Feel free to share this on this website so that other laboratories can take advantage of this.

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FAQ

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Listeria monocytogenes challengetesten are now worthy of accreditation. From now on, the Council for Accreditation (RvA) considers challenge tests for Listeria monocytogenes within microbiology to be worthy of accreditation. This gives test laboratories the opportunity to submit an accreditation or extension request for this. Biosisto has 11 Listeria Certified Reference Materials available to help you …

Challengetesten Listeria monocytogenes accreditation worthy
Tips & Tricks

For the concentration determination on the Certificate of Analysis for CRM-LM (Listeria monocytogenes), we use ALOA agar from three different suppliers. It appears that there is a difference in electivity between suppliers. The colour of the colony can vary from dark green to light green. The opaque halo around the colony is by one supplier …

Listeria monocytogenes electivity on ALOA – comparison suppliers
Tips & Tricks

Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection method Part 2: Enumeration method …

ISO 11290-1 and ISO 11290-2
Standards

Biosisto has for the process control of ISO 4833 (1 and 2) four different Certified Reference Materials available each with their own characteristics. What you choose depends on what the requirement of your laboratory is. The differences are schematically shown in the table below. Single or multi-strain CRM Accreditated Metrological Traceability of Media Performance testing …

ISO 4833 (1 and 2) selection table for choice process control
Tips & Tricks

Horizontal method for the enumeration of micro-organisms Part 1: Colony count at 30 degrees C by the pour plate technique Part 2: Colony count at 30 degrees C by the surface plating technique …

ISO 4833-1 and ISO 4833-2
Standards

1. Light sensitive When a quality control shows less growth on the DRBC plates than expected, it can happen that the DRBC plates have been in the light for too long. When DRBC is exposed to light this will result in the formulation of inhibitory compounds. Therefore keep the DRBC media in a dark and …

DRBC medium Quality issues
Tips & Tricks

CRM-LPN.BH analysis according to ISO 11731 A property value is given on the Certificate of analysis for 5 different analyses using the following methods: Without filtration (direct plating) 0,1 ml RM is diluted in 4,9 ml PFZ 0,1 ml is plated on the media With filtration, without treatment (WT) Bring 50 ml sterile water on …

Property value for Legionella on the Certificate of Analysis
Tips & Tricks

CRM-AQUA contains 3 different strains each with their growth optimums. The certificate of Analysis shows two total count method property values namely for 36°C and 22°C incubation. There is a difference in concentration is this two incubation temperatures that is caused by Pseudomonas fluorescens. This strain is not able to grow at 36°C. At 22°C …

CRM-AQUA total count 22°C versus 36°C on YEA
Tips & Tricks

Enumeration of culturable micro-organisms – Colony count by inoculation in a nutrient agar culture medium …

ISO 6222
Standards
Add 5 cfu to enrichment broth + chicken

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Without matrix With matrix …

Instruction on how to use CRM for detection methods
Tips & Tricks

Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method Part 2: Colony-count technique …

ISO 10272-1 and ISO 10272-2
Standards

Horizontal method for the detection, enumeration and serotyping of Salmonella Part 1: Detection of Salmonella spp. Part 2: Enumeration by a miniaturized most probable number technique …

ISO 6579-1 and ISO 6579-2
Standards

Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

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