There is a nomenclature change for Pseudomonas aeruginosa in Pseudomonas paraeruginosa. Phylogenomic (the evolutionary history of a particular group) and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains. The strains will be transferred from an outlier clade to a novel species Pseudomonas paraeruginosa. This change has now been implemented in the WDCM database, and …

Campylobacter spp. is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. The growth on mCCDA is part of the analysis. Besides the incubation conditions, the quality of the mCCDA medium is crucial to the analysis. There are two ISO methods available …

It is important to know that the Certified Reference Materials contain the right strain. Biosisto guarantees that the correct strain is present in the CRM. How do we do that, and what are the dangers when there are too many passages? Transferring microorganisms from one agar plate to another, known as passages, can pose risks …

Biosisto offers reference materials that match the strains mentioned in these ISO standards for cosmetics. These reference materials are essential for quality control to ensure that microbiological testing methods function correctly and provide accurate results. ISO 21149 Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria This standard specifies a method for detecting …

Pharmaceutical products are microbiologically tested on different culture media. Tests like the Growth Promotion Test (PT) must be carried out according to the European Pharmacopoeia to test if the used media are of sufficient quality. This test must be performed on each batch of culture media. Biosisto can provide ready-made Certified Reference Materials (CRM) that …

English version Working safely in a microbiological laboratory is crucial for the health of employees and the prevention of the spread of micro-organisms. Contaminations can be prevented if safety regulations are followed. Guidelines for safe working can be found in various documents: ISO 7218 Microbiology of food and animal feeding stuffs – General requirements and …

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the methods’ performance. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Interlaboratory comparison studies • Validation and verification studies CRM for dairy analyses meets ISO …

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the performance of the methods. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Performance testing of membrane filters according to ISO 7704 • Interlaboratory comparison …

Microorganisms can be divided and analyzed into the following categories and standards:   Category Treatment Min.°C Optimum.°C Max. °C Standard Psychrophilic cell – -15 10 20 – Psychrotrophic cell – -5 25 35 ISO 6222,  ISO 21527-1,  ISO 21527-2 Mesophilic cell Mesophilic spore – 10 min 80°C 10 35 45 ISO 4833-1,  4833-2 NEN 6813 …

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Enumeration pour plate method Enumeration spread plate method …

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Water filtration method …

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Without matrix With matrix …

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Qualitative CRM …

Accreditation stands for reliability. Accredited laboratories work according to standardized and validated procedures that yield reliable test results. Every food manufacturer wants to ensure their products are safe, but how can they be sure? By having their laboratory accredited, they can offer their customers greater reliability assurance. Because the accreditation process also explicitly examines the …

Biosisto has frequently asked whether we can supply CRM with a UV tag in the past year. Biosisto does not have these products. Biosisto does not have these products because working with GMMO (Genetically Modified Organisms and Micro-organisms) is linked to European regulations, and we currently do not comply with this. The laboratories that use CRM + UV …

Bacillus and Clostridium species are capable of producing spores. A spore analysis can sometimes be a difficult challenge. In most analyses, a heat treatment is necessary. The heat treatment is different per method and will kill the vegetative cells in the sample. In the case of the thermoduric analysis, it kills the not heat resistant cells. …

Process control of microbiological analyses is essential for quality control. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process control. However, not everyone realizes how useful and necessary this is. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process …

Cronobacter sakazakii is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. A part of the analysis is a streak on CCI agar. Therefore, the quality of the CCI medium is crucial to the analysis. Cronobacter sakazakii should show blue to blue-green …

Many lactobacillus species have been reclassified. This will majorly impact the market because many nutritional and probiotic supplements contain Lactobacilli. New genetic sequencing methods have led to classifying the current lactobacillus into several new groups. For Biosisto, the strain Lactobacillus plantarum in CRM-LP changes the name in Lactiplantibacillus plantarum. The new name tells you more …

The property value on the Certificate of Analysis is, in most cases, performed according to a standard like ISO or NEN. Sometimes is no standard available, and a total count of micro-organisms is displayed using a non-selective media. This is the case with quality products and CRM with low concentrations, whereby besides the standard method …

This WDCM (World Data Centre of Microorganisms) catalog was produced to enable broader and easier access to the reference strains. This collection is affiliated with the World Federation for Culture Collections (WFCC), a collection of various national culture collections. It fulfills a need expressed by these bodies for a unique system of identifiers for strains recommended …

Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

Do you want to know more about Listeria? Information about regulations and supervision, outbreaks, and disease symptoms of Listeria can be found in the articles and websites below. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) NVWA – Informatieblad 85 Interpretatiedocument microbiologische criteria (NL) RvA …

CRM-BRETT contains alive Brettanomyces bruxellensis now available! Analyses for Brettanomyces bruxellensis are very important for wineries. The presence of Brettanomyces bruxellensis indicates spoilage of the wine. Analyzing Brettanomyces bruxellensis by flow cytometry is a quick way to determine if Brettanomyces bruxellensis is present in the wine. Laboratoria investigating the wine should be reported reliable results. False-positive …