With hibernate project 2 we develop new products stored at -20°C. The new products must be homogeneous, stable for at least six months, and stable during transport. We produce microbiological (certified) reference materials for quality checks at microbiological laboratories in the context of food safety criteria and process hygiene criteria. The reference material is stored …

Process control of microbiological analyses is essential for quality control. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process control. However, not everyone realizes how useful and necessary this is. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process …

When quality managers at food manufacturing companies make smart use of the microbiological analysis results, they can enhance the accuracy of the samples’ microbiological values. Any deviations in values are also picked up sooner, which benefits food safety. How do laboratories currently work? Accredited microbiological laboratories are required to perform internal quality controls. Non-accredited laboratories …

Many factors determine the productivity of a medium. The composition must be correct, the ingredients must be good quality, the correct sterilization temperature must have been used, and the pH must be correct. If one of these factors is not in line with requirements, the enumeration may be much lower than expected. Whether or not …

Accreditation stands for reliability. Accredited laboratories work according to standardized and validated procedures that yield reliable test results. Every food manufacturer wants to ensure their products are safe, but how can they be sure? By having their laboratory accredited, they can offer their customers greater reliability assurance. Because the accreditation process also explicitly examines the …

Biosisto has frequently asked whether we can supply CRM with a UV tag in the past year. Biosisto does not have these products. Biosisto does not have these products because working with GMMO (Genetically Modified Organisms and Micro-organisms) is linked to European regulations, and we currently do not comply with this. The laboratories that use CRM + UV …

Bacillus and Clostridium species are capable of producing spores. A spore analysis can sometimes be a difficult challenge. In most analyses, a heat treatment is necessary. The heat treatment is different per method and will kill the vegetative cells in the sample. In the case of the thermoduric analysis, it kills the not heat resistant cells. …

Pharmaceutical products are microbiologically tested on different culture media. Tests like the Growth Promotion Test (PT) must be carried out according to the European Pharmacopoeia to test if the used media are of sufficient quality. This test must be performed on each batch of culture media. Biosisto can provide ready-made Certified Reference Materials (CRM) that …

Using Certified Reference Materials (CRM) daily gives a quick and easy insight into the used methods’ performance. Use Certified Reference Materials (CRM) in Quality-Control Programs for: • Daily process control • Performance testing of culture media according to ISO 11133 • Interlaboratory comparison studies • Validation and verification studies CRM for dairy analyses meets the …

Cronobacter sakazakii is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. A part of the analysis is a streak on CCI agar. Therefore, the quality of the CCI medium is crucial to the analysis. Cronobacter sakazakii should show blue to blue-green …

Many lactobacillus species have been reclassified. This will majorly impact the market because many nutritional and probiotic supplements contain Lactobacilli. New genetic sequencing methods have led to classifying the current lactobacillus into several new groups. For Biosisto, the strain Lactobacillus plantarum in CRM-LP changes the name in Lactiplantibacillus plantarum. The new name tells you more …

The property value on the Certificate of Analysis is, in most cases, performed according to a standard like ISO or NEN. Sometimes is no standard available, and a total count of micro-organisms is displayed using a non-selective media. This is the case with quality products and CRM with low concentrations, whereby besides the standard method …

Microorganisms can be divided and analyzed into the following categories and standards:   Category Treatment Min.°C Optimum.°C Max. °C Standard Psychrophilic cell – -15 10 20 – Psychrotrophic cell – -5 25 35 ISO 6222,  ISO 21527-1,  ISO 21527-2 Mesophilic cell Mesophilic spore – 10 min 80°C 10 35 45 ISO 4833-1,  4833-2 NEN 6813 …

This WDCM (World Data Centre of Microorganisms) catalog was produced to enable broader and easier access to the reference strains. This collection is affiliated with the World Federation for Culture Collections (WFCC), a collection of various national culture collections. It fulfills a need expressed by these bodies for a unique system of identifiers for strains recommended …

Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

Do you want to know more about Listeria? Information about regulations and supervision, outbreaks, and disease symptoms of Listeria can be found in the articles and websites below. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) NVWA – Informatieblad 85 Interpretatiedocument microbiologische criteria (NL) RvA …

CRM-BRETT contains alive Brettanomyces bruxellensis now available! Analyses for Brettanomyces bruxellensis are very important for wineries. The presence of Brettanomyces bruxellensis indicates spoilage of the wine. Analyzing Brettanomyces bruxellensis by flow cytometry is a quick way to determine if Brettanomyces bruxellensis is present in the wine. Laboratoria investigating the wine should be reported reliable results. False-positive …

ISO 19344 Starter cultures, probiotics, and fermented products – Quantification of lactic acid bacteria by flow cytometry. For different applications Biosisto measures alive and dead cells according to ISO 19344 protocol B using SYTO® 24 (green fluorescent cell-permeant nucleic acid stain) and PI (Propidium iodide is a red-fluorescent nuclear and chromosome counterstain. According to ISO …

Brettanomyces bruxellensis is a yeast that is part of the Ascomycota phylum and plays a key role in the production of several Belgian beers, as well as provides the wine with certain aroma’s. Brettanomyces bruxellensis is also known as Dekkara bruxellensis, where the name that is used depends on the stage in its life cycle. …

The CRM-BRETT is designed for internal quality control for the analysis of Brettanomyces bruxellensis in wine using the Cyflow BrettCount kit from Sysmex. The reference material contains alive Brettanomyces bruxellensis. With this Certified Reference Material, the entire analysis is checked for correctness, such as the work-up, staining, and the final analysis with the flow cytometry. …

  Geobacillus stearothermophilus under the microscope. Cell staining with safranine gives red cells Spore staining with malachite green gives green spores …

The source of the Biosisto strains is the NCCB*1 collection or the CCUG*2 collection. In addition, Biosisto will deposit the strain into the NCCB collection if the strain comes from a customer. You can find the strains we use at the culture bank websites, see related links below. The choice of strain(s) in Biosisto CRM products depends …

Listeria monocytogenes challenge testing is now worthy of accreditation. From now on, the Council for Accreditation (RvA) considers challenge tests for Listeria monocytogenes within microbiology to be worthy of accreditation. This gives test laboratories the opportunity to submit an accreditation or extension request for this. Biosisto has 11 Listeria Certified Reference Materials available to help …

Biosisto has for the process control of ISO 4833 (1 and 2) four different Certified Reference Materials available, each with its own characteristics. What you choose depends on what the requirement of your laboratory is. The differences are schematically shown in the table below. Single or multi-strain CRM Accreditated Metrological Traceability of Media Performance testing …