Certified Reference Materials for cosmetic analyses

ISO 11930 is a standard for evaluating the antimicrobial protection of a cosmetic product. This standard is crucial for ensuring the safety and stability of cosmetic products by determining their ability to resist microbial contamination. The purpose of this ISO 11930 is: To assess the effectiveness of preservatives in cosmetic products. To ensure the microbial …

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European Pharmacopoeia (Ph. Eur.)

Pharmaceutical products are microbiologically tested on different culture media. Tests like the Growth Promotion Test (PT) must be carried out according to the European Pharmacopoeia to test if the used media are of sufficient quality. This test must be performed on each batch of culture media. Biosisto can provide ready-made Certified Reference Materials (CRM) that …

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Working safely with micro-organisms – Veilig werken met micro-organismen

English version Working safely in a microbiological laboratory is crucial for the health of employees and the prevention of the spread of micro-organisms. Contaminations can be prevented if safety regulations are followed. Guidelines for safe working can be found in various documents: ISO 7218 Microbiology of food and animal feeding stuffs – General requirements and …

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Certified Reference Materials for dairy analyses

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the methods’ performance. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Interlaboratory comparison studies • Validation and verification studies CRM for dairy analyses meets ISO …

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Certified Reference Materials for water analyses

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the performance of the methods. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Performance testing of membrane filters according to ISO 7704 • Interlaboratory comparison …

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Total count catagories

Microorganisms can be divided and analyzed into the following categories and standards:   Category Treatment Min.°C Optimum.°C Max. °C Standard Psychrophilic cell – -15 10 20 – Psychrotrophic cell – -5 25 35 ISO 6222,  ISO 21527-1,  ISO 21527-2 Mesophilic cell Mesophilic spore – 10 min 80°C 10 35 45 ISO 4833-1,  4833-2 NEN 6813 …

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Accreditation of company laboratory inspires confidence

Accreditation stands for reliability. Accredited laboratories work according to standardized and validated procedures that yield reliable test results. Every food manufacturer wants to ensure their products are safe, but how can they be sure? By having their laboratory accredited, they can offer their customers greater reliability assurance. Because the accreditation process also explicitly examines the …

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Certified Reference Materials with a UV tag

Biosisto has frequently asked whether we can supply CRM with a UV tag in the past year. Biosisto does not have these products. Biosisto does not have these products because working with GMMO (Genetically Modified Organisms and Micro-organisms) is linked to European regulations, and we currently do not comply with this. The laboratories that use CRM + UV …

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Spore analysis – How the heat treatment van effects the analyses

Bacillus and Clostridium species are capable of producing spores. A spore analysis can sometimes be a difficult challenge. In most analyses, a heat treatment is necessary. The heat treatment is different per method and will kill the vegetative cells in the sample. In the case of the thermoduric analysis, it kills the not heat resistant cells. …

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Process control of microbiological analyses

Process control of microbiological analyses is essential for quality control. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process control. However, not everyone realizes how useful and necessary this is. Laboratories that carry out microbiological analyses for quality control are advised to carry out a daily process …

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Cronobacter sakazakii on CCI – comparison suppliers

Cronobacter sakazakii is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. A part of the analysis is a streak on CCI agar. Therefore, the quality of the CCI medium is crucial to the analysis. Cronobacter sakazakii should show blue to blue-green …

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Changes in the taxonomy of Lactobacillus

Many lactobacillus species have been reclassified. This will majorly impact the market because many nutritional and probiotic supplements contain Lactobacilli. New genetic sequencing methods have led to classifying the current lactobacillus into several new groups. For Biosisto, the strain Lactobacillus plantarum in CRM-LP changes the name in Lactiplantibacillus plantarum. The new name tells you more …

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Analyzes not linked to a standard on the Certificate of Analysis

The property value on the Certificate of Analysis is, in most cases, performed according to a standard like ISO or NEN. Sometimes is no standard available, and a total count of micro-organisms is displayed using a non-selective media. This is the case with quality products and CRM with low concentrations, whereby besides the standard method …

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Strains behind a WDCM number

This WDCM (World Data Centre of Microorganisms) catalog was produced to enable broader and easier access to the reference strains. This collection is affiliated with the World Federation for Culture Collections (WFCC), a collection of various national culture collections. It fulfills a need expressed by these bodies for a unique system of identifiers for strains recommended …

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E.coli O.157:H7 appearance on CCA, TBX and RAPID’E.coli 2 Medium

Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

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Information and articles about Listeria spp.

Do you want to know more about Listeria? Information about regulations and supervision, outbreaks, and disease symptoms of Listeria can be found in the articles and websites below. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) NVWA – Informatieblad 85 Interpretatiedocument microbiologische criteria (NL) RvA …

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Certified Reference Material – Brettanomyces bruxellensis

CRM-BRETT contains alive Brettanomyces bruxellensis now available! Analyses for Brettanomyces bruxellensis are very important for wineries. The presence of Brettanomyces bruxellensis indicates spoilage of the wine. Analyzing Brettanomyces bruxellensis by flow cytometry is a quick way to determine if Brettanomyces bruxellensis is present in the wine. Laboratoria investigating the wine should be reported reliable results. False-positive …

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ISO 19344 flow cytometry method

ISO 19344 Starter cultures, probiotics, and fermented products – Quantification of lactic acid bacteria by flow cytometry. For different applications Biosisto measures alive and dead cells according to ISO 19344 protocol B using SYTO® 24 (green fluorescent cell-permeant nucleic acid stain) and PI (Propidium iodide is a red-fluorescent nuclear and chromosome counterstain. According to ISO …

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Brettanomyces bruxellensis / Dekkara bruxellensis

Brettanomyces bruxellensis is a yeast that is part of the Ascomycota phylum and plays a key role in the production of several Belgian beers, as well as provides the wine with certain aroma’s. Brettanomyces bruxellensis is also known as Dekkara bruxellensis, where the name that is used depends on the stage in its life cycle. …

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Instruction how to use CRM-BRETT using the Cyflow BrettCount kit from Sysmex

The CRM-BRETT is designed for internal quality control for the analysis of Brettanomyces bruxellensis in wine using the Cyflow BrettCount kit from Sysmex. The reference material contains alive Brettanomyces bruxellensis. With this Certified Reference Material, the entire analysis is checked for correctness, such as the work-up, staining, and the final analysis with the flow cytometry. …

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Biosisto - Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

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