Campylobacter spp. is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell.
The growth on mCCDA is part of the analysis. Besides the incubation conditions, the quality of the mCCDA medium is crucial to the analysis.

There are two ISO methods available for food analyses, which this medium is mentioned:
– ISO 10272-1 Detection method
ISO 10272-2 Enumeration method

Modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) is a blood-free medium to isolate Campylobacter spp. selectively.
mCCDA contains a lot of ingredients. These ingredients must be of good quality and quantity for Campylobacter to grow well.

mCCDA (Modified Charcoal Cefoperazone Deoycholate Agar) medium contains the following ingredients:

  • Nutrient broth – nitrogen, vitamin, and mineral source
  • Casein hydrolysate – nitrogen, vitamin, and mineral source
  • Bacteriological charcoal – absorbs toxic substances and metabolites
  • Sodium deoxycholate – inhibits gram-negative enteric bacteria except Campylobacter spp.
  • Ferrous sulfate – iron(II) sulfate, which neutralizes toxic oxygen products
  • Sodium pyruvate – neutralizes toxic oxygen products
  • Amphotericin – inhibits yeast and fungi
  • Cefoperazone – inhibits gram-negative enteric bacteria except Campylobacter spp.
  • Agar
  • Water
    Final pH 7.4 ± 0.2

Incubation conditions
44h±4h at 41.5°C±1°C under microaerobic conditions.

Appearance on mCCDA 
Typical colonies are greyish on mCCDA, often with a metallic sheen. They are flat and moist and tend to spread, but they tend to spread less on drier agar surfaces.

Electivity on mCCDA of different media suppliers
Research shows a difference in selectivity between suppliers using the same Certified Reference Material – Campylobacter jejuni (CRM-CJ) under the same incubation conditions.
All three suppliers declare that the mCCDA is made according to ISO 10272, but its appearance is diverse.
Sometimes, the colonies are significantly smaller than the other. Smaller colonies can be missed because they are not characteristic enough.
Most likely, the basic ingredients have different origins. This could explain the differences.

If you have doubts about the selectivity of the colony on mCCDA, it is worth trying another mCCDA media supplier to test their selectivity on the mCCDA.

***  We would like to hear about your experiences with mCCDA. Leave your comment below  ***







We will respond to your message by email as soon as possible.
Comments specificly relevant for this article and other visitors will be published in this section. Questions that also apply to other articles are published in the F.A.Q. section.

Comment on this article

Your email address will not be published. Required fields are marked *

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Biosisto - Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

Biosisto b.v.
Martin Luther Kingweg 3
9403 PA Assen, The Netherlands