ISO 9308-1

Enumeration of Escherichia coli and coliform bacteria – Part 1: Membrane filtration method for waters with low bacterial background flora

 

Posted - Author Annemarie
Recommended CRM for process control
  • WDCM 00013
Process control CRM-ECO.00013BL - CCA Process control CRM-ECO.00013BL - LSA
Can also be used for performance testing
  • WDCM 00006
Process control CRM-CFR.00006BL - CCA Process control CRM-CFR.00006BL - LSA
Can also be used for performance testing
    CRM-AQUA
  • Escherichia coli
  • Pseudomonas fluorescens
  • Enterococcus faecalis
  • WDCM 00013
  • Wild strain
  • WDCM 00087
Process control CRM-AQUA - CCA Process control CRM-AQUA - LSA
-
Alternative CRM’s
    CRM-COFA
  • Escherichia coli
  • Enterococcus faecium
  • WDCM 00179
  • WDCM 00178
Process control alternatives CRM-COFA - CCA Process control alternatives CRM-COFA - LSA
 -
CCA
  • WDCM 00013
Performance testing CRM-ECO.00013BL - CCA Performance testing CRM-ECO.00013BL - LSA Performance testing CRM-ECO.00013BL - CCA Performance testing CRM-ECO.00013BL - LSA

PR ≥ 0,7
Dark-blue to violet colonies

  • WDCM 00006
Performance testing CRM-CFR.00006BL - CCA

PR ≥ 0,7
Pink to red colonies

  • WDCM 00009

Total or partial inhibition

  • WDCM 00024
Performance testing CRM-PAE.00024ML - LSA Performance testing CRM-PAE.00024ML - LSA

Colourless colonies

Performance testing is according to ISO 9308-1:2014

CRM-ECO.00012BL is suitable for TBA (Tryptone Bile Agar) filtration method with incubation at 44°C.

CRM-ECO.00012BL is suitable for LSA (Membrane Lauryl Sulphate Agar) filtration method with incubation at 36

 

 

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Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

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Chromogenic Medium for the enumeration, without confirmation, of E. coli and other coliforms in water samples. …

RAPID’E.coli 2 for Water Testing, alternative method for ISO 9308-1
Standards

Biosisto - Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

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