CRM-AQUA contains 3 different strains each with their growth optimums.
The certificate of Analysis shows two total count method property values namely for 36°C and 22°C incubation. There is a difference in concentration is this two incubation temperatures that is caused by Pseudomonas fluorescens. This strain is not able to grow at 36°C. At 22°C Pseudomonas fluorescens will grow perfectly.
The concentration at 22°C is a sum of concentration of Escherichia coli, Enterococcus faecalis and Pseudomonas fluorescens.
The concentration at 36°C is the sum of the concentration of Escherichia coli and Enterococcus faecalis.