It is important to know that the Certified Reference Materials contain the right strain. Biosisto guarantees that the correct strain is present in the CRM. How do we do that, and what are the dangers when there are too many passages? Transferring microorganisms from one agar plate to another, known as passages, can pose risks …

Pharmaceutical products are microbiologically tested on different culture media. Tests like the Growth Promotion Test (PT) must be carried out according to the European Pharmacopoeia to test if the used media are of sufficient quality. This test must be performed on each batch of culture media. Biosisto can provide ready-made Certified Reference Materials (CRM) that …

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the methods’ performance. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Interlaboratory comparison studies • Validation and verification studies CRM for dairy analyses meets ISO …

Using Certified Reference Materials (CRM) daily gives quick and easy insight into the performance of the methods. Use Certified Reference Materials (CRM) in your Quality Control Program for: • Daily process control • Performance testing of culture media according to ISO 11133 • Performance testing of membrane filters according to ISO 7704 • Interlaboratory comparison …

Microorganisms can be divided and analyzed into the following categories and standards:   Category Treatment Min.°C Optimum.°C Max. °C Standard Psychrophilic cell – -15 10 20 – Psychrotrophic cell – -5 25 35 ISO 6222,  ISO 21527-1,  ISO 21527-2 Mesophilic cell Mesophilic spore – 10 min 80°C 10 35 45 ISO 4833-1,  4833-2 NEN 6813 …

Biosisto has frequently asked whether we can supply CRM with a UV tag in the past year. Biosisto does not have these products. Biosisto does not have these products because working with GMMO (Genetically Modified Organisms and Micro-organisms) is linked to European regulations, and we currently do not comply with this. The laboratories that use CRM + UV …

Bacillus and Clostridium species are capable of producing spores. A spore analysis can sometimes be a difficult challenge. In most analyses, a heat treatment is necessary. The heat treatment is different per method and will kill the vegetative cells in the sample. In the case of the thermoduric analysis, it kills the not heat resistant cells. …

Cronobacter sakazakii is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. A part of the analysis is a streak on CCI agar. Therefore, the quality of the CCI medium is crucial to the analysis. Cronobacter sakazakii should show blue to blue-green …

Many lactobacillus species have been reclassified. This will majorly impact the market because many nutritional and probiotic supplements contain Lactobacilli. New genetic sequencing methods have led to classifying the current lactobacillus into several new groups. For Biosisto, the strain Lactobacillus plantarum in CRM-LP changes the name in Lactiplantibacillus plantarum. The new name tells you more …

This WDCM (World Data Centre of Microorganisms) catalog was produced to enable broader and easier access to the reference strains. This collection is affiliated with the World Federation for Culture Collections (WFCC), a collection of various national culture collections. It fulfills a need expressed by these bodies for a unique system of identifiers for strains recommended …

Escherichia coli O.157:H7 and Escherichia coli O.157 (non-VTEC) give a false negative result for Escherichia coli on the CCA, TBX, and RAPID’E.coli 2 Medium because the colonies do not appear characteristic. Colonies of Escherichia coli appear on CCA (Chromogenic Coliform Agars) according to ISO 9308-1 as dark-blue to violet colonies. However, Escherichia coli O.157:H7 doesn’t do …

Do you want to know more about Listeria? Information about regulations and supervision, outbreaks, and disease symptoms of Listeria can be found in the articles and websites below. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) NVWA – Informatieblad 85 Interpretatiedocument microbiologische criteria (NL) RvA …

CRM-BRETT contains alive Brettanomyces bruxellensis now available! Analyses for Brettanomyces bruxellensis are very important for wineries. The presence of Brettanomyces bruxellensis indicates spoilage of the wine. Analyzing Brettanomyces bruxellensis by flow cytometry is a quick way to determine if Brettanomyces bruxellensis is present in the wine. Laboratoria investigating the wine should be reported reliable results. False-positive …

Brettanomyces bruxellensis is a yeast that is part of the Ascomycota phylum and plays a key role in the production of several Belgian beers, as well as provides the wine with certain aroma’s. Brettanomyces bruxellensis is also known as Dekkara bruxellensis, where the name that is used depends on the stage in its life cycle. …

  Geobacillus stearothermophilus under the microscope. Cell staining with safranine gives red cells Spore staining with malachite green gives green spores …

The source of the Biosisto strains is the NCCB*1 collection or the CCUG*2 collection. In addition, Biosisto will deposit the strain into the NCCB collection if the strain comes from a customer. You can find the strains we use at the culture bank websites, see related links below. The choice of strain(s) in Biosisto CRM products depends …

Biosisto has for the process control of ISO 4833 (1 and 2) four different Certified Reference Materials available, each with its own characteristics. What you choose depends on what the requirement of your laboratory is. The differences are schematically shown in the table below. Single or multi-strain CRM Accreditated Metrological Traceability of Media Performance testing …

CRM-AQUA contains 3 different strains each with their growth optimums. The certificate of Analysis shows two total count method property values namely for 36°C and 22°C incubation. There is a difference in concentration is this two incubation temperatures that is caused by Pseudomonas fluorescens. This strain is not able to grow at 36°C. At 22°C …

CRM-LPN.BH analysis according to ISO 11731 A property value is given on the Certificate of analysis for 5 different analyses using the following methods: Without filtration (direct plating) 0,1 ml RM is diluted in 4,9 ml PFZ 0,1 ml is plated on the media With filtration, without treatment (WT) Bring 50 ml sterile water on …

Many aspects play a key role in the analysis of ISO 11731, such as filters, washing the filter and media. To verify all aspects of the analysis, Biosisto created new products that are specially designed for this analysis. The products CRM-LPN.BH, CRM-LAN.00106BL and CRM-LPN.00107BH can be used for media control, quality control, learning purposes, verification …

It is essential to know if your Salmonella method still does what it should. Reliable results are essential. False-negative or false-positive results must be reduced to a minimum. Daily process control using certified reference material is a way to get insight into the method’s performance characteristics. Biosisto has five types of Certified Reference Materials (CRM) Salmonella …

Many factors determine the productivity of a medium. The composition must be correct, the ingredients must be good quality, the correct sterilization temperature must have been used, and the pH must be correct. If one of these factors is not in line with requirements, the enumeration may be much lower than expected. Whether or not …