Handy tips, tricks, tutorials and general information about (working with) Biosisto reference materials.
ISO 19344 Starter cultures, probiotics, and fermented products – Quantification of lactic acid bacteria by flow cytometry. For different applications Biosisto measures alive and dead cells according to ISO 19344 protocol B using SYTO® 24 (green fluorescent cell-permeant nucleic acid stain) and PI (Propidium iodide is a red-fluorescent nuclear and chromosome counterstain. According to ISO …
Brettanomyces bruxellensis is a yeast that is part of the Ascomycota phylum and plays a key role in the production of several Belgian beers, as well as provides the wine with certain aroma’s. Brettanomyces bruxellensis is also known as Dekkara bruxellensis, where the name that is used depends on the stage in its life cycle. …
The CRM-BRETT is designed for internal quality control for the analysis of Brettanomyces bruxellensis in wine using the Cyflow BrettCount kit from Sysmex. The reference material contains alive Brettanomyces bruxellensis. With this Certified Reference Material, the entire analysis is checked for correctness, such as the work-up, staining, and the final analysis with the flow cytometry. …
Geobacillus stearothermophilus under the microscope. Cell staining with safranine gives red cells Spore staining with malachite green gives green spores …
The source of the Biosisto strains is the NCCB*1 collection or the CCUG*2 collection. In addition, Biosisto will deposit the strain into the NCCB collection if the strain comes from a customer. You can find the strains we use at the culture bank websites, see related links below. The choice of strain(s) in Biosisto CRM products depends …
Listeria monocytogenes challenge testing is now worthy of accreditation. From now on, the Council for Accreditation (RvA) considers challenge tests for Listeria monocytogenes within microbiology to be worthy of accreditation. This gives test laboratories the opportunity to submit an accreditation or extension request for this. Biosisto has 11 Listeria Certified Reference Materials available to help …
Biosisto has for the process control of ISO 4833 (1 and 2) four different Certified Reference Materials available, each with its own characteristics. What you choose depends on what the requirement of your laboratory is. The differences are schematically shown in the table below. Single or multi-strain CRM Accreditated Metrological Traceability of Media Performance testing …
Listeria monocytogenes is a serious food pathogen. If you are looking for this pathogen, you want to detect this bacteria down to the last cell. The quality of the ALOA medium used is a crucial part of the analysis. Research shows that there is a difference in electivity between suppliers. The color of the colony …
CRM-AQUA contains 3 different strains each with their growth optimums. The certificate of Analysis shows two total count method property values namely for 36°C and 22°C incubation. There is a difference in concentration is this two incubation temperatures that is caused by Pseudomonas fluorescens. This strain is not able to grow at 36°C. At 22°C …
Two DRBC quality issues you should know. This article may help you troubleshoot quality issues with the DRBC medium. DRBC medium is described in ISO 21527-1 for the isolation and enumeration of yeast and moulds. DRBC is the abbreviation of Dichloran Rose-Bengal Chloramphenicol Agar. Rose-bengal photo-oxidizes to form toxic compounds. Store DRBC plates in the …
CRM-LPN.BH analysis according to ISO 11731 A property value is given on the Certificate of analysis for 5 different analyses using the following methods: Without filtration (direct plating) 0,1 ml RM is diluted in 4,9 ml PFZ 0,1 ml is plated on the media With filtration, without treatment (WT) Bring 50 ml sterile water on …
Many aspects play a key role in the analysis of ISO 11731, such as filters, washing the filter and media. To verify all aspects of the analysis, Biosisto created new products that are specially designed for this analysis. The products CRM-LPN.BH, CRM-LAN.00106BL and CRM-LPN.00107BH can be used for media control, quality control, learning purposes, verification …
There is a lot of information on the Certificate of Analysis. What the information tells you is explanted of the Certificate of Analysis over here More information about accreditation you will find at What is the difference between accreditated and not accreditated CRM? …
Process Control or Internal Quality Control (IQC) Process control also known as internal quality control or first-line control, is an important tool to monitor the whole analyzing process, day after day. All facets in the analysis are included with this quality check. The process control can be performed using Certified Reference Materials (CRM’s). To monitor …
Definitions according to ISO 11133 Performance of culture medium Response of a culture medium to challenge by test organisms under defined conditions expressed in one or more functions such as productivity, selectivity, selectivity and specificity. The incubation conditions of the media are mentioned in the corresponding analyses standard. 1. Productivity of culture medium Level of …
It is essential to know if your Salmonella method still does what it should. Reliable results are essential. False-negative or false-positive results must be reduced to a minimum. Daily process control using certified reference material is a way to get insight into the method’s performance characteristics. Biosisto has five types of Certified Reference Materials (CRM) Salmonella …
Many factors determine the productivity of a medium. The composition must be correct, the ingredients must be good quality, the correct sterilization temperature must have been used, and the pH must be correct. If one of these factors is not in line with requirements, the enumeration may be much lower than expected. Whether or not …
When quality managers at food manufacturing companies make smart use of the microbiological analysis results, they can enhance the accuracy of the samples’ microbiological values. Any deviations in values are also picked up sooner, which benefits food safety. How do laboratories currently work? Accredited microbiological laboratories are required to perform internal quality controls. Non-accredited laboratories …
With hibernate project 2 we develop new products stored at -20°C. The new products must be homogeneous, stable for at least six months, and stable during transport. We produce microbiological (certified) reference materials for quality checks at microbiological laboratories in the context of food safety criteria and process hygiene criteria. The reference material is stored …
Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.
Biosisto b.v.
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9403 PA Assen, The Netherlands
www.biosisto.com
