ISO 21528-2

Horizontal methods for the detection and enumeration of Enterobacteriaceae — Part 2: Colony-count method

Posted - Author Annemarie
Recommended CRM for process control
  • WDCM 00013
Process control CRM-EC - VRBG
Can also be used for performance testing
  • WDCM 00012
Process control CRM-ECO.00012MM - VRBG
Can also be used for performance testing
  • WDCM 00006
Process control CRM-CFREU - VRBG
-
Alternative CRM’s
    CRM-BLES
  • Escherichia coli
  • Staphylococcus aureus
  • Bacillus cereus
  • Lactiplantibacillus plantarum
  • WDCM 00013
  • WDCM 00034
  • Wild strain
  • Wild strain 2
Process control alternatives CRM-BLES - VRBG
 -
VRBG
  • WDCM 00012
Performance testing CRM-ECO.00012MM - VRBG

PR ≥ 0,5
Pink to red colonies with or without precipitation halo

    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031
Performance testing CRM-ST - VRBG

PR ≥ 0,5
Pink to red colonies with or without precipitation halo

  • WDCM 00009

Total inhibition (0)

Glucose agar / O-F medium with overlay
  • WDCM 00013

Positive reaction:
Yellow colour

  • WDCM 00025

Negative reaction:
Growth, but no yellow colour development

Oxidase reagent
  • WDCM 00025

Positive reaction:
Mauve, violet, purple or dark blue colour in the reaction time

  • WDCM 00013

Negative reaction:
No colour change in the reaction time

Lactiplantibacillus plantarum, previously known as Lactobacillus plantarum.

We will respond to your message by email as soon as possible.
Comments specificly relevant for this article and other visitors will be published in this section. Questions that also apply to other articles are published in the F.A.Q. section.

Comment on this article

Your email address will not be published. Required fields are marked *

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Analyse

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Enumeration pour plate method Enumeration spread plate method …

Instructions on how to use CRM for enumeration methods
Tips & Tricks
Streak out

The CRMs are easy to use. Follow the photos step by step. Details, such as concentration, can be found on the Certificate of Analysis Qualitative CRM …

Instructions on how to use qualitative CRM
Tips & Tricks

The 3M Petrifilm Enterobacteriaceae is a sample-ready-culture-medium system …

Petrifilm Enterobacteriaceae (EB), alternative method for ISO 21528-2
Standards

For the enumeration of Enterobacteriaceae without the need for confirmation. …

RAPID’Enterobacteriaceae, alternative method for ISO 21528-2
Standards

Biosisto - Quality in Control

Microbiological laboratories should be able to receive reliable analysis without much overhead. A microbiological analyst must be able to focus on the primary activities, for example analysis of the sample material. Quality control should support the analyst in a logical and simple manner. By using modern information technology, process control and quality assurance are designed smarter and more effective.

Biosisto b.v.
Martin Luther Kingweg 3
9403 PA Assen, The Netherlands
www.biosisto.com

BiosistoStandard