Recommended CRM for process control
    CRM-SE
  • Salmonella enterica subsp. enterica serotype Enteritidis
  • Wild strain
-
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031
Can also be used for performance testing
Alternative CRM’s
  • Wild strain
-
  • Wild strain
-
  • Wild strain
-
BPW
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Turbidity (1-2)

MKTTN
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

> 10 colonies on XLD

  • WDCM 00013

Partial inhibition ≤100 colonies on TSA

  • WDCM 00087

< 10 colonies on TSA

MSRV
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Grey-white turbid zone extending out from inoculated drop(s). After 24-48h, the turbid zone(s) will be (almost) fully migrated over the plate
Possible extra: characteristic colonies subculturing on XLD

  • WDCM 00013

Possible growth at the place of the inoculated drop(s) without a turbid zone

  • WDCM 00087

No growth

RVS
  • WDCM 00031

Salmonella typhimurium + Escherichia coli + Pseudomonas aeruginosa
> 10 colonies on XLD
Characteristic colonies according to each medium (see ISO 6579)

  • WDCM 00025

see for criteria CRM-ST

  • WDCM 00013

For the multi-strain control – See criteria CRM-ST

For the single stain control – Partial inhibition ≤  colonies on TSA

  • WDCM 00087

< 10 colonies on TSA

XLD
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Good growth (2)
Colonies with a black centre and a light transparent zone of reddish colour due to the colour change of the medium

  • WDCM 00012

Growth or partial inhibition (0-1)
Yellow colonies

 

  • WDCM 00009

Total inhibition (0)

Triple Sugar Iron agar (TSI)
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Butt
Yellow: Glucose fermented
Black: Formation of hydrogen sulphite
Bubbles or cracks: Gas formation

Slant surface
Yellow: Lactose and/or sucrose utilized

  • WDCM 00127

Butt
Yellow: Glucose fermented
No formation of hydrogen sulphite
No gas formation

Slant surface
red or unchanged: Lactose and sucrose not utilized

  • WDCM 00025

Butt
Red or unchanged: Glucose not fermented

Slant surface
red or unchanged: Lactose and sucrose not utilized

Urea agar
  • WDCM 00023

Positief reaction: Liberation of ammonia with colour change to rose/rose-pink/deep cerise

  • WDCM 00012

Negative reaction: No liberation of ammonia, no change of colour

L-Lysine decarboxylation medium (LDC)
    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Positive reaction: Medium remains purple after incubation and is turbid

  • WDCM 00012

Negative reaction: Medium changes from purple to yellow

Saline solution with toluene and β-Galactosidase reagent
  • WDCM 00012

Positive reaction: Yellow colour

  • WDCM 00023

Negative reaction: No colour change

Indol reaction medium / reagent
  • WDCM 00012

Positive reaction: Formation of red ring within 10 min

    CRM-ST
  • Salmonella enterica subsp. enterica serotype Typhimurium
  • WDCM 00031

Negative reaction: Yellow / brown ring within 10 min

CRM-SAG (Salmonella agona) is a lactose positive strain.

For performance testing of Urea agar and L-Lysine decarboxylation medium, Proteus mirabilis WDCM 00023 is not available in the Biosisto collection. If you need this strain, please get in touch with us.

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